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EC number: 618-522-8
CAS number: 9030-09-5
A study was conducted to assess the inhibitory effect of alpha-amylase
on the growth of the unicellular green alga Desmodesmus subspicatus,
Strain No. CCAP 276/20.
The study was conducted in accordance with OECD Guideline for Testing of
Chemicals No. 201 and EEC Methods for the Determination of Ecotoxicity,
EEC Directive 67/458 Annex VIII, Part C (87/302/EEC).
Algal cultures exposed to five test concentrations of alpha-amylase plus
one untreated control were incubated on an orbital shaker under
continuous illumination at 24 ± 1°C for 72 hours. Growth was
monitored daily by measuring the absorbance of each culture at 665 nm.
The following values were derived from the data:
EbC50(72h): 24 mg/l
corresponding to 2.5 mg active enzyme protein (aep)/L
ErC50(24-72h): 49 mg/l
corresponding to 5.2 mg aep/L
NOEC 72h for AUC (Area under the curve): 12.5 mg/L corresponding to 1.3
NOEC for the 24 -72 hour growth rate: 25 mg/L corresponding to 2.6 mg
Alpha-amylase was inhibitory to the growth of Desmodesmus
subspicatus at concentrations in excess of 12.5 mg/L corresponding to
1.3 mg active enzyme protein (aep)/mL. The EbC50 (72h) value is 24 mg/L
and the ErC50 (24-72h) is 49 mg/L corresponding to 2.5 and 5.2 mg aep/L,
Due to the similarity between enzymes, similar results are
expected for cyclomaltodextrin glucanotransferase.
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