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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 479-310-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Very similar to the appropriate guidelines
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Salmonella-Escherichia coli / Mammalian-Microsome reverse
mutation screening study. Ames, BN, McCann, J. Yamasaki, E.
"Methods for detecting carcinogens and mutagens with the
Slamonella/Mammalian microsome mutagenicity test", Mutation
Research, Vol. 31:347-364(1975). - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- E-AF098T (Batch No. T# 176-3), a white powder. Stored at room temp, dry, closed container.
Method
- Target gene:
- Histidine and Tryptophan
Species / strain
- Species / strain / cell type:
- bacteria, other: Salmonella typhimurium TA1535, TA1537, TA98, TA100 and E. coli WP2 uvrA
- Details on mammalian cell type (if applicable):
- not relevant
- Additional strain / cell type characteristics:
- other: Strains TA98 and TA1537 were capable of detecting frameshift mutagens, strains TA100 and TA1535 are capable of detecting base-pair substitution mutagens
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 homogenate, liver microsomal enzymes, commercially purchased produced from Spague-Dawley rats that have been injected with Arocolor 1254 (200 mg/ml in corn oil)
- Test concentrations with justification for top dose:
- Concentration range in the test (without metabolic activation): 1, 3.33, 10, 33.3, 100, 333, 1000, 3330, 5000 µg/plate
Concentration range in the test (without metabolic activation): 10, 33.3, 100, 333, 1000, 3330, 5000 µg/plate - Vehicle / solvent:
- Solvent: DMSO
- Details on test system and experimental conditions:
- See attached document of test system
- Evaluation criteria:
- Criteria for a positive response: 1) For tester strains TA98, TA100 and WP2urA: For a test article to be considered positive, it must produce at least a 2-fold
increase in the mean revertants per plate of at least one of these tester strains over the mean revertants per plate of the appropriate vehicle control.
2) For tester strains TA1535 and TA1537: For a test article to be considered positive, it must produce at least a 3-fold increase in the mean revertants per
plate of at least one of these tester strains over the mean revertants per plate of the appropriate vehicle control. - Statistics:
- See above "Evaluation criteria".
Results and discussion
Test results
- Species / strain:
- other: as specified above
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- (> 5000 µg/plate)
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- (> 5000 µg/plate)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Observations:
No positive increase in mean number of revertants per plate
observed with any of the tester strains in the presence or
absence of S9 mix. A 3.75 increase in the mean number of
revertants per plate was observed with tester strain TA 1537
in the absence of S9 mix, but not meeting the criteria for
a postive eveluation. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
See attached Tables (Summary of Results-tables 1 & 2)
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
The results indicate E-AF098T was negative in the Salmonella - E. coli/Mammalian- Microsom Reverse Mutation Screening Study under the conditions, and according to the criteria, of the study. - Executive summary:
The results indicate E-AF098T was negative in the Salmonella - E. coli/Mammalian- Microsom Reverse Mutation Screening Study under the conditions, and according to the criteria, of the study.
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