Reaction product of 2-hydroxyethylacrylate with imidazole

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

guideline study according to GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction product of 2-hydroxyethylacrylate with imidazole

EC Number:

926-135-3

Cas Number:

1181222-25-2

Molecular formula:

not applicable

IUPAC Name:

Reaction product of 2-hydroxyethylacrylate with imidazole

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: batch: 0015609877
- Expiration date of the lot/batch: Expiry date: 18 Aug 2017
- Purity test date:

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test conditions

Details on test conditions:

Incubation time: 3 hours
Test temperature: 18.9 – 20.8 °C
Test vessels: Glas-beakers (nominal volume 1 L)
Test volume: 500 mL
Synthetic medium: 16 mL/test vessel of 100-fold concentrated OECD
medium
Oxygen concentration during aeration: > 2 mg/L
Oxygen concentration immediately
before measurement:
> 7 mg/L
Duration of the measurement of
oxygen consumption:
About 8 - 10 min
Sludge concentration in the test vessel: 1.5 g/L Dw

Test system: municipal Activated sludge
Reason for the selection of the test system: required by the test guidelines
Age: 1 day
Origin: aeration tank of the wastewater treatment plant of Mannheim, Germany
Collection of the test system: 25 Jan 2017
Arrival in the test facility: 25 Jan 2017
After arrival of the activated sludge suspension in the test facility the suspension was sieved
with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over
night at room temperature. At the next day the sludge suspension was washed once with
drinking water and the suspension was adjusted to 3 g/L Dw.
3.3. TEST GROUPS AND CONCENTRATIONS
Test concentrations: 1000, 500, 250, 125, 62.5 mg/L as nominal concentration
based on test substance without correction of purity and blank
controls.
100, 10, 1 mg/L as nominal concentration based on reference
substance.
Test replicates: 6 replicates for the control,
3 replicates for each test substance concentration,
2 replicates for each reference substance concentration.
Reason for selection of
test concentrations:
According to the test guidelines, at least 5 concentrations in a
geometric series with a separation factor of ≤ 3.2 should be
used, preferably encompassing the range from 10 – 80%
inhibition .

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Effect concentrations 2

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The test substance was tested in an OECD 209 guiodelien test for toxicity again microorganisms. The EC10 and EC50 were monitored to be > 1000 mg/l.