Chlorodiphenylphosphine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study available in EPA (TSCA8e submission file). The study was not performed under GLP. The data are well documented and the data acceptable

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Primary skin irritation was determined according to the proposed FDA revision of the test for primary skin irritants published in the Federal Register 37, no. 244, Dec. 19, 1972.
The proposed test differs from the procedure described in the Hazardous Substances Labelling Act, Part 191.11, Chapter, Title 21 in that irritation is determined after a 4-hour exposure period rather than after the proviously required 24-hour exposure period. In the proposed test, readings are made 4, 24, and 48 hours after treatment. Animals are to be retained for observation 96 hours after initial application. Any delayed necrosis will be reported, but the data will not be used in determination of irritation indices. A corrosive substance is defined as a material that causes tissue destruction within 48 hours after application on any of the six intact sites with or without similar destruction on the abraded areas.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Chlorodiphenylphosphine

EC Number:

214-093-2

EC Name:

Chlorodiphenylphosphine

Cas Number:

1079-66-9

Molecular formula:

C12H10ClP

IUPAC Name:

diphenylphosphinous chloride

Test material form:

liquid

Test animals

Species:

rabbit

Test system

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Controls:

no

Duration of treatment / exposure:

4 hours

Observation period:

96 hours

Number of animals:

6

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

4

Max. score:

4

Reversibility:

other: corrosive at 4h

Remarks on result:

other: observation at 4h, 24h and 48h

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

4

Max. score:

4

Reversibility:

other: corrosive at 4h

Remarks on result:

other: observation at 4h, 24h and 48h

Irritant / corrosive response data:

All test rabbits exhibited extensive eschar with severe edema.

Any other information on results incl. tables

Skin irritation index

N°	Skin	Erythema – Eschar observation			Edema observation			Sum Total	Score
		4h	24h	48	4h	24h	48
1	Intact	4	4	4	4	4	4	48	8
	Abraded	4	4	4	4	4	4
2	Intact	4	4	4	4	4	4	48	8
	Abraded	4	4	4	4	4	4
3	Intact	4	4	4	4	4	4	48	8
	Abraded	4	4	4	4	4	4
4	Intact	4	4	4	4	4	4	48	8
	Abraded	4	4	4	4	4	4
5	Intact	4	4	4	4	4	4	48	8
	Abraded	4	4	4	4	4	4
6	Intact	4	4	4	4	4	4	48	8
	Abraded	4	4	4	4	4	4
Primary irritant Score									8

Score = Sum of individual valus for each rabbit divided by 6

Applicant's summary and conclusion

Interpretation of results:

Category 1B (corrosive) based on GHS criteria

Conclusions:

Based on the findings of the in vivo study with rabbits, the test substance is considered to be corrosive to the skin