1,2-bis(chloromethyl)benzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Richtlinie 84/449/EWG vom 25. April 1984

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Specific details on test material used for the study:

Purity: ca. 98%

Test solutions

Vehicle:

yes

Details on test solutions:

The test item was mixed with the vehicle and water and homogenised using an Ultra-Turrax and by ultrasonic treatment.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 3 - 3.5 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.5 - 3.3 cm, 0.18 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food:
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Test temperature:

Test groups: 21.3 - 23.0 °C
Control groups: 21.0 - 23.0

pH:

Test groups: 7.1 - 8.1
Control groups: 7.4 - 8.2

Dissolved oxygen:

Test groups: 6.0 - 8.8 mg/L
Control groups: 5.4 - 9.3 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 0.35 - <= 0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 0.35 - <= 0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 d

Dose descriptor:

LC100

Effect conc.:

0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

From this study LC50 for 1,2-Bis(chloromethyl)benzene was determined to be in the range of 0.35 - 0.5 mg/L