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EC number: 615-086-0 | CAS number: 70225-05-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Conducted according OECD TG 471. GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,2,4-Benzenetricarboxylic acid, tris(2-ethylhexyl) ester
- IUPAC Name:
- 1,2,4-Benzenetricarboxylic acid, tris(2-ethylhexyl) ester
- Reference substance name:
- Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate
- EC Number:
- 222-020-0
- EC Name:
- Tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate
- Cas Number:
- 3319-31-1
- Molecular formula:
- C33H54O6
- IUPAC Name:
- tris(2-ethylhexyl) benzene-1,2,4-tricarboxylate
- Details on test material:
- CAS #3319-31-1; 1,2,4-Benzenetricarboxylic acid, tris(2-ethylhexyl) ester
Constituent 1
Constituent 2
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- without S9: 0, 313, 625, 1250, 2500, 5000 ug/plate (five strains)
with S9: 0, 313, 625, 1250, 2500, 5000 ug/plate (five strains) - Vehicle / solvent:
- Acetone
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone, DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9 mix, 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (TA100, WP2, TA98) Sodium azide (TA1535) 9-Aminoacridine (TA 1537); With S9 mix, 2- Aminoanthracene (five strains)
- Details on test system and experimental conditions:
- Prior to assay initiation, a toxicity pretest was performed using tester strain TA100. Based on these results, the doses for the final assay were determined. Each chemical was tested initially at half-log dose intervals up to a dose that elicited toxicity, or to a dose immediately below one which was toxic in the preliminary toxicity test. Chemicals that were not toxic were tested, with few exceptions, to a maximum dose of 10 mg/plate. In the definitive assay, each of the five strains was dosed with either the test substance; a vehicle control (DMSO); or a nontreated control and a positive control. The test mixture containing the tester strain and test substance with or without S9 was added to the surface of petri dishes containing Vogel-Bonner medium. The S-9 (9,000g supernatant) fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared as described previously [Haworth et al, 1983]. The S-9 mixes were prepared immediately prior to use and contained either 10% or 30% S-9; occasionally, other levels were used. The histidine-independent colonies that formed on the plates were counted following a two-day incubation at 37°C. Positive controls were as follows: 2 aminoanthracene (all strains with S9); sodium azide (without S9, TA1535, TA100), 4-nitro-ophenylenediamine (without S9, TA98) and 9-aminoacridine (without S9, TA 97, TA1537). There were 3 plates/dose group/strain/treatment. The test results were verified by repeating the assay. If the results were negative, they were repeated first without S9 and then with 30% S9.
Species/Strain: Salmonella typhimurium TA100, TA1535, TA98, TA1537; Escherichia coli WP2 uvrA
Positive controls:
Without S9 mix, 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (TA100, WP2, TA98) Sodium azide (TA1535)
9-Aminoacridine (TA 1537)
With S9 mix, 20 Aminoanthracene (five strains)
S9: Rat liver, induced with phenobarbital and 5,6-benzoflavone - Evaluation criteria:
- Chemicals were judged to be mutagenic if the test results produced a dose-related, reproducible increase in histidine revertants over control. It was not a requirement for mutagenic responses to reach two-fold over background.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- no genotoxic effects were observed at the highest dose tested (5000 ug/plate)
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- Toxicity was not observed up to 5000 ug/plate in five strains with and without metabolic activation (S9 mix).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- no genotoxic effects were observed at the highest dose tested (5000 ug/plate)
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- Toxicity was not observed up to 5000 ug/plate in five strains with and without metabolic activation (S9 mix).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation at the highest dose tested (5000 ug/plate)
negative without metabolic activation at the highest dose tested (5000 ug/plate)
No genotoxic effects were observed at the highest dose tested (5000 ug/plate) in all five strains of bacteria, with or without metabolic activation.
Classification as a genotoxic agent is not warranted under the new Regulation (EC) 1272/2008 on classification, labeling and packaging of substances and mixtures (CLP) or under Directive 67/548/EEC for dangerous substances and Directive 1999/45/EC for preparations. - Executive summary:
The test material did not induce a statistically signifiant increase in revertant numbers in any of the test strains, either in the absence or in the presence of S-9. This study was therefore considered to have provided no indication of any test material mutagenic activity.
Classification as a genotoxic agent is not warranted under the new Regulation (EC) 1272/2008 on classification, labeling and packaging of substances and mixtures (CLP) or under Directive 67/548/EEC for dangerous substances and Directive 1999/45/EC for preparations.
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