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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
Version / remarks:
13 Apr 2004
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-difluoroaniline
EC Number:
206-687-5
EC Name:
2,4-difluoroaniline
Cas Number:
367-25-9
Molecular formula:
C6H5F2N
IUPAC Name:
2,4-difluoroaniline

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
56 d

Test conditions

Nominal and measured concentrations:
Solvent control, water control, 7.8, 15.6, 31.3, 62.5. 125, 250, 500 and 1000 mg pure metabolite /kg soil
Solvent control, water control, 8.0, 16.0, 32.0, 63.9, 127.8, 255.6, 511.2, and 1022.5 mg test item/kg soil

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
500 other: mg pure metabolite/kg soil dry weight
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
>= 500 other: mg pure metabolite/kg soil dry weight
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: weight and feeding activity
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
125 other: mg pure metabolite/kg soil dry weight
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No mortality was observed in any treatment group except of the concentration of 1000 mg pure metabolite/kg (28% mortality).
No effects on body weight could be observed after 4 weeks exposure.
No statistically significant effects on reproduction were observed up to the concentration of 125 mg pure metabolite/kg soil.
The feeding activity in all the treated groups was comparable to the control.
The NOEC (28 d) for weight changes and feeding activity of the earthworm Eisenia fetida was ≥ 500 mg pure metabolite/kg soil, i.e. the highest concentration tested.
The NOEC (28 d) for mortality was 500 mg pure metabolite/kg soil. The NOEC (56 d) for reproduction was 125 mg pure metabolite/kg.