Oxo(propan-2-olato)aluminium

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 to 28 February 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The acute toxicity to Daphnia study was undertaken as a bridging study to demonstrate equivalence between the Target substance (XP 473) and the Source substance (aluminum, benzoate C16-18-fatty acids complexes; CAS 94166-87-7) for the read-across of other data. A full discussion of read-across and supporting data is given in the attached justification in the endpoint for acute toxicity to fish.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 13, 2004.

Deviations:

yes

Remarks:

Mixing and settling periods for sample preparation selected by study monitor.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Oxo(propan-2-olato)aluminium

EC Number:

270-365-0

EC Name:

Oxo(propan-2-olato)aluminium

Cas Number:

68425-65-0

Molecular formula:

C3 H7 Al O2

IUPAC Name:

oxo(propan-2-olato)aluminium

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The concentration of XP 473 was determined by measuring the concentration of total aluminum in all control and test substance treatment solutions at 0 and 48 hours. The 0-hour samples were collected from the parent solutions and the 48-hour samples were collected after combining replicate test solutions by treatment.

Test solutions

Vehicle:

no

Details on test solutions:

Test solutions prepared by water-accommodated fraction (WAF), prepared by adding test substance to water to achieve 100 mg/L loading rate. Solution stirred for 72 h at room temperature while being shielded from light. Mixing was followed by 24 h settling period. Then the solution was filtered first through glass wool, and then through a 0.2 µm filter. The filtered solution was used as the 100 mg/L nominal loading rate test solution, and also for dilution to lower concentrations. The control solution was prepared in a similar way, but with dilution water only.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna neonates (<24-hours old) were obtained from an in house daphnid culture. All daphnids were cultured in a temperature-controlled area at approximately 20°C. During the culture period, the daphnids were fed a suspension of the algal species Raphidocelis subcapitata (formerly Selenastrum capricornutum) at least once a day supplemented by an artificial diet consisting of a wheat grass, salmon starter, and yeast suspension (YCT). Less than 24 hours old neonates for the definitive test were collected from in-house culture containing adults that were approximately 27 days old. The adults were considered acceptable with no signs of stress, disease or physical damage. Since the culturing and testing environmental parameters were equivalent (i.e., temperature, dilution water, and lighting), no acclimation period was necessary. Test daphnids were not fed during the test.

Study design

Test type:

static

Water media type:

freshwater

Remarks:

The dilution water used for the definitive test was natural surface water collected on-site at the testing facility, and tested to verify it was free of contaminants that might interfere with test results.

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

180 mg CaCO3/L

Test temperature:

20 °C

pH:

8.5 to 8.6

Dissolved oxygen:

5.3 to 8.9 mg/L (62 to 105% saturation)

Conductivity:

692 μS/cm

Nominal and measured concentrations:

Nominal, based on water-accommodated fractions (WAF): 0 (control), 6.3, 13, 25, 50, and 100 mg total product per litre.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

79.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Reported statistics and error estimates:

The 24-hour EC50 value was estimated to be >100 mg TP/L, the highest concentration tested. The 48-hour EC50 value was estimated to be 79.4 mg TP/L, with 95% confidence limits of 70.4 and 89.4 mg TP/L. The 48 hour NOEC was 50 mg TP/L, based on the nominal XP 473 loading rate concentrations and the lack of statistically significant effects at this, and all lower test substance concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 value was estimated to be 79.4 mg/L (WAF).