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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017 to 2018
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
Fish were exposed, in groups of seven, to an aqueous solution of the test item at nominal concentrations of 3.13, 6.25, 12.5, 25 and 50% v/v saturated solution for a period of 96 hours at a temperature of 14 to 15 C under semi static test conditions. The test item solution was prepared by stirring an excess (100 mg/L) of test item in test water using a propeller stirrer at approximately 1500 rpm for 24 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Sartorius Sartopore filter, first approximate 2 liters discarded in order to pre condition the filter) to produce a 100% v/v saturated solution of the test item. The number of mortalities and any sub lethal effects of exposure in each test and control vessel were determined 1, 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Nominal and measured concentrations:
Nominal: 3.13, 6.25, 12.5, 25, 50 %v/v
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.9 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
mortality
Details on results:
Analysis of the freshly prepared test media at 0 and 72 hours showed measured test concentrations to range from 0.71 to 12 mg/L. A decline in measured test concentration was observed in the old or expired test media at 24 and 96 hours to between 0.38 and 7.6 mg/L and hence it was considered appropriate to calculate the results based on the time-weighted mean measured test concentration only in order to give a “worst case” analysis of the data.
Validity criteria fulfilled:
yes
Conclusions:
The 96-hr LC50 value for rainbow trout was determined to be 2.9 mg/L based on geometric mean measured test concentrations.
Executive summary:

An OECD 203 study was conducted with MCS-1562 exposed to the rainbow trout (Oncorhynchus mykiss). The 96 -hr LC50 value was determined to be 2.9 mg/L as was based on geometric mean measured concentrations.

Description of key information

An OECD 203 study was conducted with MCS-1562 exposed to the rainbow trout (Oncorhynchus mykiss). The 96 -hr LC50 value was determined to be 4.0 mg/L as was based on geometric mean measured concentrations.

Key value for chemical safety assessment

LC50 for freshwater fish:
4 mg/L

Additional information