(ethyl 3-oxobutyrato-O1',O3)bis(propan-2-olato)aluminium

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

8 March 2004 and 5 April 2004.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

Remarks:

The test material rapidly hydrolyses in aqueous media and some of the hydrolysis products may be volatile, so the Manometric Respirometry Test was considered best.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

451-250-6

EC Name:

-

Cas Number:

569318-35-0

Molecular formula:

C11 H19 Al O5

IUPAC Name:

Aluminium 1, 3-butanediol ethyl acetoacetate complexes

Test material form:

solid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

A mixed population of sewage sludge micro-organisms was obtained from the final effluent stage of the Severn Trent Water PLC sewage treatment plant, which treats predominantly domestic sewage. The sample of effluent was filtered through course filter paper and maintained on aeration in a temperature controlled room at 21 °C.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

As biodegradation progresses, the micro-organisms convert oxygen to carbon dioxide which is absorobed into ethanolamine, causing a net reduction in gas pressure with in the sample flask. The pressure reduction triggers an electrolytic process, generating oxygen and restoring the pressure in the sample flask. The magnitude and duration of the current is proportional to the amount of oxygen supplied to the microorganisms. The data on the oxygen is recorded on computer.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

88

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

76

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

42

Sampling time:

7 d

Details on results:

10 day window validation criterion passed. The substance is considered readily biodegradable.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The substance is considered readily biodegradable.