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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

delta-3 -Carene is readily biodegradable according to the criteria of Guideline OECD 301 D.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A ready biodegradability study was performed to assess the biotic degradation of alpha-pinene. Test method was designed to be compliant with OECD Guideline for Testing of Chemicals No. 301 D, "Ready biodegradability – Closed Bottle Test" (1), referenced as Method C.4-E of Commission Regulation No. 440/2008 amended by Commission Regulation (EU) 2016/266 (2) and with ISO 10634 (7).

Test item delta-3 -carene was biodegraded by 79.3% at day 28. The pass level of 60% was reached after less than 7 days (approximately 5 days) upon achieving 10% biodegradation. Therefore, delta-3 -carene is considered as readily biodegradable.

The result of this key study is proposed as end point value for the chemical safety assessment.