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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
96 hours
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Justification for type of information:
Marine species performed to US guidelines. Mixture (as manufactured) tested and results in report relate to mixture (possible to extrapolate results for active ingredient)
Dose levels high enough to confirm classification
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Semi-static sea-water study with 48 hour renewal
GLP compliance:
yes
Specific details on test material used for the study:
Stable at normal temperature and pressure
Soluble in water
Clear, yellow, viscous liquid; MSDS states clear amber Mild
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
Synthetic seawater used for both holding and testing is prepared by dissolving Hawaiian Marinemix seasalts in deionized, St. Louis municipal, water
Test organisms (species):
Cyprinodon variegatus
Details on test organisms:
The fish had a mean total length of 1.65 cm and a mean weight of 0.057 grams. Biological loading was 0.19 g/1.
Test type:
semi-static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h
pH:
6. 0 - 7.6 (test concentrations) 7.8 for negative control
Dissolved oxygen:
Reduced to 5.3 mg/l in higher concentrations over duration of exposure, but not sufficient to impact onthe outcome
Salinity:
The synthetic seawater used for both holding and testing is prepared by dissolving Hawaiian Marinemix seasalts in deionized, St. Louis municipal, water.
Nominal and measured concentrations:
Nominal 0, 250, 500, 1000, 2000, 4000 mg/l on 40% material as supplied (up to 1600 mg/l actives)
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 560 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 1 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Contains ca 40% actives
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
Contains ca 40% actives
Basis for effect:
mortality
Details on results:
The 96 hour No Effect Concentration (NOEC) is l000 mg/I
Validity criteria fulfilled:
yes
Conclusions:
Toxic to fish at high concentrations.
Toxicity thresholds sufficiently high to mean that classification is not required

Description of key information

Figure used for CSR

Key value for chemical safety assessment

LC50 for freshwater fish:
560 mg/L

Additional information