Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Sep 09, 2016 - Jan 09, 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Taurine
EC Number:
203-483-8
EC Name:
Taurine
Cas Number:
107-35-7
Molecular formula:
C2H7NO3S
IUPAC Name:
2-aminoethanesulfonic acid
Test material form:
solid: crystalline
Specific details on test material used for the study:
Name: Art. 808616
Synonym: Taurine
Colour: White crystalline solid
Expiry Date: Sep 30, 2020
Storage Conditions: At 20 ± 5 °C in the dark

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampling times 0, 24, 48 and 72 hours
Duplicate Samples


The samples were allowed to thaw to room temperature. They were then shaken well and treated with ultrasound for 1 minute to obtain homogenous samples. The samples were already diluted with acetonitrile by factor 1.25 directly after sampling. The samples were centrifuged (13,000 rpm, 3 minutes) before analysis.
Fortified samples and analytical blank control samples:
The samples were shaken well and were diluted with acetonitrile by factor 1.25. The samples were centrifuged (13,000 rpm, 3 minutes) before analysis.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata (KORSHIKOV), Strain No. 61.81 SAG, formerly known as Selenastrum capricornutum, and recently renamed as Raphidocelis subcapitata (KORSHIKOV)
- Source (laboratory, culture collection): The algae were supplied by the „Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen", 37073 Göttingen, Germany.
- Method of cultivation: The algae were cultivated in the laboratories of ibacon under standardised conditions according to the test guidelines. The algae culture used for this test was from an axenic strain transferred into liquid medium kept under aseptic condition in closed vessels with an additional carbon source to enable algae growth.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Hardness:
0.24 mmol/L
Test temperature:
22.3-22.6°C
pH:
pH was adjusted with 1 M HCl to pH 8.1.
Nominal and measured concentrations:
nominal: 100 mg test item/L


At the start of the test 103% of the nominal test concentration 100 mg test item /L were found. After 72 hours test duration, 103% of the nominal value (100mg test item/L) was determined. During the test the algae were exposed to a mean of 103% of nominal. Therefore, all reported results refer to nominal concentrations.
Details on test conditions:
According to guideline
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: Yield
Details on results:
The influence of Taurine on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static limit test. The 72-hour EyC50 > 100 mg test item/L, and the 72-hour ErC50 value was > 100 mg test item/L. The 72-hour NOEyC was determined to be ≥ 100 mg test item/L and the associated 72-hour LOEyC was > 100 mg test item/L. The 72-hour NOErC was determined to be ≥ 100 mg test item/L and the associated 72-hour LOErC was > 100 mg test item/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The influence of Art. 808616 (Taurine) on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static limit test. The 72-hour EyC50 and ErC50 were determined to be > 100 mg test item/L. The 72-hour NOEyC was determined to be ≥ 100 mg test item/L and the associated 72-hour LOEyC was > 100 mg test item/L. The 72-hour NOErC was determined to be ≥ 100 mg test item/L and the associated 72-hour LOErC was > 100 mg test item/L.
Executive summary:

The influence of Art. 808616 (Taurine) on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static limit test. The 72-hour EyC50 and ErC50 were determined to be > 100 mg test item/L. The 72-hour NOEyC was determined to be ≥ 100 mg test item/L and the associated 72-hour LOEyC was > 100 mg test item/L. The 72-hour NOErC was determined to be ≥ 100 mg test item/L and the associated 72-hour LOErC was > 100 mg test item/L.