1,1'-[ethane-1,2-diylbis(thio)]bisbenzene

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 May 2011 to 26 June 2011

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

none

Vehicle:

yes

Remarks:

10 mL acetone

Details on preparation and application of test substrate:

- Method of application to filter paper (if used):
- Method of mixing into soil (if used):
- Method of application to soil surface (if used): in solvent
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): aceton
- Concentration of vehicle in test medium (stock solution and final test solution): 10mL
- Evaporation of vehicle before use:yes

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

The earthworms were purchased from Shanghair, Minhang Earthworm Farm as adults, at least two months old with clitellum and with an indivdual weight of 0.3 to 0.6 g.

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

20 +/- 2°C

pH:

6.0 +/- 0.5

Moisture:

35%

Details on test conditions:

TEST SYSTEM
- Test container (material, size):1L glassware
- Amount of soil or substrate:
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 2
- No. of replicates per control: 2
- No. of replicates per vehicle control: 2

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location:
- Pesticide use history at the collection site:
- Collection procedures:
- Sampling depth (cm):
- Soil texture (if natural soil)
- % sand:
- % silt:
- % clay:
- Soil taxonomic classification:
- Soil classification system:
- Composition (if artificial substrate): 10% sphagum peat, 20% kaolin clay and 70% industrial san
- Organic carbon (%):
- Maximum water holding capacity (in % dry weigth):
- CEC:
- Pretreatment of soil:
- Storage (condition, duration):
- Stability and homogeneity of test material in the medium:

OTHER TEST CONDITIONS
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Nominal and measured concentrations:

1000 mg/kg nominal

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 for earthworms was reported as >1,000 kg/mg dry soil.

Executive summary:

An OECD 207 Earthworm acute toxicity study was conducted. A preliminary study indicated that the defintive study could be conducted as a Limit Test. The reported 14-d LC50 value was greater than the highest nominal test concentration of 1,000 mg/kg.

Description of key information

An OECD 207 acute earthworm toxicity test, conducted as a Limit Test report a 14 -day LC50 value of 1,000 mg/kg dry soil weight.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw