4,4'-trimethylenedipiperidine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04 October 2017 to 30 November 2017

Reliability:

1 (reliable without restriction)

Justification for type of information:

Reliable experimental study following OECD 202 guidelines (2004) and EC No. 440/2008 Method C.2 adopted 2008 and GLP standards.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

yes

Remarks:

appearance of test item preparations not documented at 0 h; test temperature recorded at start of test was 23 ˚C rather than 18-22˚C; positive control study overdue for twice per year rate; deviations did not affect integrity or validity of the study.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

2008

Deviations:

yes

Remarks:

appearance of test item preparations not documented at 0 h; test temperature recorded at start of test was 23 ˚C rather than 18-22˚C; positive control study overdue for twice per year rate; deviations did not affect integrity or validity of the study.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test substance is listed as DIPIP (CAS # 16898-52-5). The test substance was stored at room temperature in the dark.

Analytical monitoring:

yes

Details on sampling:

T

Vehicle:

not specified

Details on test solutions:

A nominal amount of test item (200 mg) was dissolved in test water with the aid of ultrasonication for 10 minutes and the volume adjusted to 2 liters to give the 100 mg/L test concentration. The pH of the 100 mg/L test concentration was adjusted to 8.0 prior to a series of dilutions being made to give further test concentrations of 10, 18, 32 and 56 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test was carried out using first instar Daphnia magna derived from in-house laboratory cultures.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

Temperature of 21 °C to 23 °C

pH:

pH of water 7.7 to 8.1

Dissolved oxygen:

Dissolved oxygen 8.4 to 8.7 mg O2/L

Nominal and measured concentrations:

Daphnids were exposed to an aqueous solution of the test item at nominal concentrations of 10, 18, 32, 56 and 100 mg/L.

Details on test conditions:

Following preliminary range-finding tests, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test item at nominal concentrations of 10, 18, 32, 56 and 100 mg/L for 48 hours at a temperature of 21 °C to 23 °C under static test conditions. The number of immobilized daphnia were recorded after 24 and 48 hours.

Reference substance (positive control):

yes

Remarks:

A positive control (Envigo Study Number HP54JV) used potassium dichromate as the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

28 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Remarks:

The test was considered valid since none of the control daphnids showed immobilization or other signs of disease or stress and the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.

Conclusions:

The test substance did induce acute immobilisation of Daphnia magna at nominal concentrations of 32 mg/L and 56 mg/L after 48 h of exposure. The 48-h NOEC was 18 mg/L, the 48-h LOEC was 32 mg/L and the 48-hr EC50 was 28 mg/L.

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the freshwater invertebrate Daphnia magna following OECD Guideline 202. The test substance induced acute immobilisation of Daphnia magna at nominal concentrations of32 mg/L and 56 mg/Lafter 48 h of exposure. The 48-h NOEC was 18 mg/L, the 48-h LOEC was 32 mg/L and the 48-hr EC50 was 28 mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

28 mg/L