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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, but no mention of GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: U.S. Department of Transportation, 49 CFR 173.240
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
109-89-4
IUPAC Name:
109-89-4
Constituent 2
Chemical structure
Reference substance name:
4-methylpyridine
EC Number:
203-626-4
EC Name:
4-methylpyridine
Cas Number:
108-89-4
Molecular formula:
C6H7N
IUPAC Name:
4-methylpyridine
Details on test material:
Lot # 20310. Sample received 1983-05-11.

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Housing: stainless teel with elevated wire mesh flooring, 1 rabbit/cage.
- Diet (e.g. ad libitum): Wayne 15% rabbit ration
- Water (e.g. ad libitum): tap water ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°F): 60-75 degrees F
- Humidity (%): 40-45%
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 1983-06-09 To: 1983-06-11

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Controls:
no
Amount / concentration applied:
0.5 ml portion of test substance was applied to the skin covering 1 square inch. Surgical gauze patches measuring not less than 1" x 1" and two single layers thick were placed over the treated area and an impervious material was wrapped snugly around the trunk of the animal to hold the patch in place.
Duration of treatment / exposure:
4 hour contact period. At this time, the site was examined, then washed off with water to avoid continued exposure.
Observation period:
Readings were made at 4, 24 and 48 hours after application.
Number of animals:
6
Details on study design:
Corrosion was considered to have resulted if the material in contact with skin had caused destruction or irreversible alteration of the tissue. Tissue destruction was considered to have occurred if, at any of the readings, tere was ulceration or necrosis. Thissue destruction was not considered to have occurred if merely sloughingof the epidermis, erythema, edema or fissuring was evident.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: objective evaluation of irritation/corrosion
Basis:
animal #1
Time point:
other: 24
Remarks on result:
other: moderate erythema, slight edema, no corrosion
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #1
Time point:
other: 48
Remarks on result:
other: severe erythema, no edema, no corrosion
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #2
Time point:
other: 24
Remarks on result:
other: severe erythema, slight edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #2
Time point:
other: 48
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #3
Time point:
other: 24
Remarks on result:
other: severe erythema, slight edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #3
Time point:
other: 48
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #4
Time point:
other: 24
Remarks on result:
other: severe erythema, slight edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #4
Time point:
other: 48
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #5
Time point:
other: 24
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #5
Time point:
other: 48
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #6
Time point:
other: 24
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritation parameter:
other: objective assessment of irritation/corrosion
Basis:
animal #6
Time point:
other: 48
Remarks on result:
other: severe erythema, no edema, Corrosive
Irritant / corrosive response data:
5 of 6 animals displayed evidence of corrosion

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information Criteria used for interpretation of results: other: U.S. Department of Transportation
Conclusions:
4-Methylpyridine was corrosive to the skin of rabbits after a 4 hour exposure, according to a U.S. Department of Transportation protocol.