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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Similar to a guideline study but preceeds establishment of guidelines and GLP.

Data source

Reference
Reference Type:
review article or handbook
Title:
Unnamed
Year:
1972

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 433 draft (Acute Inhalation Toxicity: Fixed Concentration Procedure) (not officially approved)
Version / remarks:
preceeds establishment of guideline
GLP compliance:
not specified
Test type:
fixed concentration procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
4-methylpyridine
EC Number:
203-626-4
EC Name:
4-methylpyridine
Cas Number:
108-89-4
Molecular formula:
C6H7N
IUPAC Name:
4-methylpyridine
Details on test material:
no data

Test animals

Species:
rat
Strain:
not specified
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Duration of exposure:
> 0.15 - < 2.5 h
Concentrations:
Average concentration was 17.5 g/m3 or 17500 mg/m3.
No. of animals per sex per dose:
6
Control animals:
not specified
Details on study design:
Exposure occurred in a stainless steel chamber of 35 L capacity.
A concentrated atmosphere of vapour was generated by passing a stream of air through 109 g of substance in a 500 ml Erlenmyer flask. Vapours passed through a 1-liter bottle to remove droplets and then into the exposure chamber. Air flow through the sample was 4.0 liters/min as measured through a calibrated rotameter. No supplemental air was introduced as oxygen content was sufficient.

Temperature of the chamber: 78 degrees F or 25.6 degrees C.
Relative humidity of the chamber: 70%.

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LC100
Effect level:
17 500 mg/m³ air (nominal)
Based on:
test mat.
Remarks on result:
other: Equivalent to 4075 ppm
Mortality:
All animals died within 2.5 hours.
Clinical signs:
other: Increasing weakness, labored breathing, collapse and death.
Gross pathology:
hemorrhagic lungs and hyperemic livers.

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category III
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
An acute inhalation toxicity (limit) test was conducted in a whole body protocol using male rats with 4-methylpyridine at a concentration of 17500 mg/m3. All rats died within 2.5 h of exposure. The LC50 is less than 17500 mg/m3 or 4075 ppm.