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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
CAS No.: 078567-28-9; Batch No.: WDJ 1853D-3; Purity: weight percentage about 100 %;
Analytical monitoring:
yes
Details on sampling:
Sampling schedule of chemical analysis
Control: at 0 and 48 hours
Test concentrations: at 0 and 48 hours
Vehicle:
no
Details on test solutions:
Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9): 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin. This standard dilution water is used for both, the maintenance of the test animals and the preparation of stock and test solutions of the test substance.

Pretreatment oe the test substance
To give the desired series oftest concentrations, the test substance was added to 1 litre of dilution water for each test concentration, then treated with an ultra turrax for 60 seconds at 8000 rpm and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration using folded filters of pore size 7-12 Ilm.
Test organisms (species):
Daphnia magna
Details on test organisms:
Name: Daphnia magna STRAVS, parthenogenetic females
Source: Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1°C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semiquantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
14.8°dH (= 264.2 mg/I CaC03)
Test temperature:
mean +/- 1 °C (in the temperature range 18-22 °C)
Nominal and measured concentrations:
Test concentration/s: (nominal) 25, 50 and 100 mg/I
Details on test conditions:
Exposure conditions
Test vessels: 50 ml glass beakers holding 10 neonates in 20 ml of test medium
Experimental design: 3 test concentrations plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period
Method of initiation: neonates were placed in prepared media
Aeration: none
Photoperiod 16h light: 8 h dark
Method of administration: direct weighing
Criteria of effects: the criterion of adverse effects used in this study was the substance-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EL0
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
ca. 58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The results are expressed in terms of effective loadings (EL) at 24h and 48h. Considering the heterogeneous composition ofthe test substance, Water Accommodated Fractions (= WAFs) were tested. The results of the TOC determinations reflect a rough impression only of exposure conditions over time. No informations are available on a potential correlation between effective loadings and measured TOC values.
Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the nominal EL50 of the test substance in Daphnia magna was determined to be 58 mg/L.
Executive summary:

A study was conducted to determine the short term toxicity of the test substance to Daphnia magna STRAUS under static conditions according to EU Method C.2, in compliance with GLP. The Daphnia were exposed to a range of nominal concentrations: 25, 50 and 100 mg/L. The test substance was dispersed in water, mixed with an ultra turrax, a magnetic stirrer and finally filtered. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. Under the study conditions, the following values were determined: Highest test concentration resulting in 0% immobilisation (EC 0 48 h): 25 mg/L. Lowest test concentration resulting in 100% immobilisation (EC 100 48 h): > 100 mg/L. 40% and 90% immobilization was observed after 48 h at loading rates of 50 and 100 mg/L respectively. The results are expressed in terms of effective loadings (EL) at 24 h and 48 h. Under the study conditions, the nominal EL50 of the test substance in Daphnia magna was determined to be 58 mg/L (Bruns, 2002).

Description of key information

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
58 mg/L

Additional information

A study was conducted to determine the short term toxicity of the test substance to Daphnia magna STRAUS under static conditions according to EU Method C.2, in compliance with GLP. The Daphnia were exposed to a range of nominal concentrations: 25, 50 and 100 mg/L. The test substance was dispersed in water, mixed with an ultra turrax, a magnetic stirrer and finally filtered. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. Under the study conditions, the following values were determined: Highest test concentration resulting in 0% immobilisation (EC 0 48 h): 25 mg/L. Lowest test concentration resulting in 100% immobilisation (EC 100 48 h): > 100 mg/L. 40% and 90% immobilization was observed after 48 h at loading rates of 50 and 100 mg/L respectively. The results are expressed in terms of effective loadings (EL) at 24 h and 48 h. Under the study conditions, the nominal EL50 of the test substance in Daphnia magna was determined to be 58 mg/L (Bruns, 2002).