[No public or meaningful name is available]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 08, 2001 to March 12, 2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

No analytical dose verification

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct application of the test substance into the medium (synthetic water as per ISO guideline) at 100 mg/L, mixing with ultra turrax for 60 s at 8000 rpm, stirred for 24 h with a magnetic stirrer. Undissolved substance removed by filtration through a 7-12 µm filter.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Bio International BV (The Netherlands)
- Age at study initiation (mean and range, SD): ca. 7 months
- Length at study initiation (length definition, mean, range and SD): 2.5-3.5 cm

Test type:

static

Water media type:

freshwater

Remarks:

Synthetic freshwater as per ISO guideline

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20.7 - 22.1

pH:

7.6 - 7.9

Dissolved oxygen:

92.6 - 104.1%

Nominal and measured concentrations:

- Nominal: 100 mg/L

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LL0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, the 96 h LL0 of the test substance in zebra fish was determined to be greater than 100 mg/L WAF.

Executive summary:

A study was conducted to determine the short term toxicity of the test substance to zebra fish (Danio rerio) according to EU Method C.1. The test substance was dispersed in the medium (synthetic water as per ISO guideline) at 100 mg/L, mixed with an ultra turrax for 60 s at 8000 rpm, then stirred for 24 h with a magnetic stirrer. Undissolved substance was removed by filtration through a 7 -12 µm filter. Ten fish were exposed to the water accommodated fraction (WAF) or to synthetic water (controls) for 96 h. Mortality and signs of toxicity were recorded. No analytical dose verification of the test medium was conducted. There was no mortality and fish swimming behavior was normal throughout the study. Under the study conditions, the 96 h LL0 of the test substance in zebra fish was determined to be greater than 100 mg/L WAF (Bruns, 2001).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

A study was conducted to determine the short term toxicity of the test substance to zebra fish (Danio rerio) according to EU Method C.1. The test substance was dispersed in the medium (synthetic water as per ISO guideline) at 100 mg/L, mixed with an ultra turrax for 60 s at 8000 rpm, then stirred for 24 h with a magnetic stirrer. Undissolved substance was removed by filtration through a 7 -12 µm filter. Ten fish were exposed to the water accommodated fraction (WAF) or to synthetic water (controls) for 96 h. Mortality and signs of toxicity were recorded. No analytical dose verification of the test medium was conducted. There was no mortality and fish swimming behavior was normal throughout the study. Under the study conditions, the 96 h LL0 of the test substance in zebra fish was determined to be greater than 100 mg/L WAF (Bruns, 2001).