reaction mass of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2H -isothiazol-3-one

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Version / remarks:

Part B

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

other: Amber liquid

Details on test material:

- Name of test material (as cited in study report):Kathon(TM) 886F biocide

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. 6111L019

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Soluble and stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dilution in water
- Final dilution of a dissolved solid, stock liquid or gel: 62.5, 125, 250, 500 and 1000 mg a.i./L

OTHER SPECIFICS: Purity of test material was 14.2%, of which 10.8% CMIT and 3.4% MIT

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 62.5, 125, 250, 500 and 1000 mg ai/L
- Sampling method: Dry weights were determined from plant shoots collected on day 21
- Sample storage conditions before analysis: Not described

Test substrate

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of application to soil surface (if used): Foliar application
- Controls: negative control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Water
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable
- Evaporation of vehicle before use: Not applicable

Test organisms

1

Species:

Trifolium pratense

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Red clover

2

Species:

Oryza sativa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: Rice

3

Species:

Brassica napus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: Oilseed rape

Study design

Test type:

vegetative vigour test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

Mean: 22.98 C

pH:

7.5

Moisture:

Mean: 74.76 %

Details on test conditions:

TEST SYSTEM
- Testing facility: Greenhouse
- Test container (type, material, size): Plastic pots (10 cm x 11 cm)
- Amount of soil: Not described
- Method of seeding: Not described
- No. of seeds per container: Not described
- No. of plants (retained after thinning): 5
- No. of replicates per treatment group: 6
- No. of replicates per control: 6

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Not described
- Pesticide use history at the collection site: Not described
- Collection procedures: Not described
- Sampling depth (cm): Not described
- Soil texture (if natural soil)
- % sand: 53%
- % silt: 30%
- % clay: 17%
- Soil taxonomic classification: Sandy loam
- Soil classification system: Not described
- Organic carbon (%): 1.1 %
- Maximum water holding capacity (in % dry weigth): Not described
- CEC: Not described
- Pretreatment of soil: Not described
- Storage (condition, duration): Not described

GROWTH CONDITIONS
- Photoperiod: 14 hour photoperiod
- Light source: Not described
- Light intensity and quality: Mean moles photosynthetically active radiation: 13.2
- Day/night temperatures: Mean: 22.98 °C
- Relative humidity (%): Mean: 74.76 %
- Wind velocity: Not described
- Air exchange rate: Not described
- Watering regime and schedules: By sub-irrigation once or twice weekly
- Water source/type: Well water
- Volume applied: ca. 200 L/ha
- Interval of applications: Single application
- Method of application: Spray
- Any pest control method/fertilization (if used): Seeds were not pretreated with insecticides or fungicides.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observation periods: 0, 7, 14 and 21 days, seedling condition. Dry weights were determined from plant shoots collected on day 21.

VEHICLE CONTROL PERFORMED: yes

Nominal and measured concentrations:

Nominal: 0, 62.5, 125, 250, 500 and 1000 mg a.i./L
Mean measured:

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Species:

Brassica napus

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: highest concentration tested

Effect concentrations 2

Key result

Species:

Oryza sativa

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: highest concentration tested

Effect concentrations 3

Key result

Species:

Trifolium pratense

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: highest concentration tested

Details on results:

VEGETATIVE VIGOUR
- Percent seed germination: Not examined
- Plant height: No effects
- Dry weight: No effects
- Abnormal seed development or appearance: Not described
- Lesions: Not described
- Swelling: Not described
- Loss of turgour: Not described
- Discoloration: Not described
- Unusual leaf/plant shape or size: Not described
- Dead plants: No effects

INJURY RATING SYSTEM: No apparent signs of treatment-related phytotoxicity to any of the three species

Reported statistics and error estimates:

Statistical analyses were used to evaluate effects of TS application on plant biomass and survival. Analyses were performed on data from the test using the Dunnett option of the GLM (general linear model) procedure of SAS version 8 (SAS Institute, Inc. 1999. SAS Proprietary Software Version 8, Cary, North Carolina, USA). Significance was determined at the level of 0.05 and 0.01 (Dunnett’s test, p < 0.05, p < 0.01). Test data were evaluated to determine the LOEC and NOEC for plant biomass. Dunnett’s test was used to establish the LOEC and NOEC by determining which treatment groups differed significantly (p < 0.05, p < 0.01) from the control group.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

21d NOEC = 1000 mg a.i./L, the highest concentration tested

Executive summary:

OECD Draft Guideline 208, Part B, terrestrial non-target plant test: vegetative vigour test with analytical confirmation of dosing solutions. No apparent signs of treatment-related phytotoxicity to any of the three species. 21d NOEC = 1000 mg a.i./L, the highest concentration tested.