3-(Hydroxymethyl)-1-pentene

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06.05. - 10.10.2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Version / remarks:

2006

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The test item (50.34 mg) was dissolved in acetonitrile and made up to the mark in a 50 mL
volumetric flask to prepare a stock solution of 1007 mg/L. Thereof, 1 mL were diluted to 10 mL
using acetonitrile/water (v/v, 9/1) to obtain a working solution with a concentration of 101 mg/L.
Defined volumes of this working solution were diluted with acetonitrile/water (v/v, 9/1) to obtain
calibration solutions of the test item in the range of 0.101 to 10.1 mg/L. These solutions were
used to calibrate the GC-MS-system.

Vehicle:

no

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus CHODAT
- Strain: 86.81 SAG
- Source: Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

0.24 mmol/L

Test temperature:

23 - 24 °C

pH:

7.9 - 8.4

Nominal and measured concentrations:

Nominal concentrations: 4.6, 10, 22, 46, 100 mg/L
Measured concentrations of the test concentrations of 46 and 100 mg/L were between 87 and 103% of the nominal values at the start and the end of the test. The test item was stable in the test media over the test period of 72 hours.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50-mL Erlenmeyer flasks
- Initial cells density: 5000 cells/mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO 14442, NaHCO3 was increased by 200 mg/L to 250 mg/L
- Intervals of water quality measurement: pH: at start and end of test, temperature daily

OTHER TEST CONDITIONS
- Light intensity and quality: approximately 7000 Lux (range: 6500 to 7450 Lux)

TEST CONCENTRATIONS
- Range finding study:
- Test concentrations: 4.6, 10, 22, 46 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

potassium dichromate, tested twice a year

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95% CI: 46-69 mg/L

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

The microscopic examination of the algal cells at the end of the test showed no difference between the algae growing at the nominal test concentration of 100 mg/L and the algal cells in the control. The shape and size of the algal cells were obviously not affected by the test item up to at least this concentration.

Results with reference substance (positive control):

latest positive control test performed in 2008: 72-hour EC50 for the growth rate 0.69 mg/L

Validity criteria fulfilled:

yes

Conclusions:

A test on growth inhibition to Scenedesmus subspicatus was conducted according to OECD 201. Analytical measurements revealed that the test substance concentration was >80% during the test. After 72 h of exposure, both EC10 and EC50 were determined to be >100 mg/L, related to the growth rate.

Executive summary:

The influence of the test item on the growth of the freshwater green
algal species Scenedesmus subspicatus was investigated in a 72-hour static test according to the OECD Guideline 201 (2006) and the EU Commission Directive 92/69/EEC, C.3.
The nominal concentrations of the test item of 4.6, 10, 22, 46 and 100 mg/L were tested in parallel with a control. The measured concentrations of the test item in the test media of the test concentrations of 46 and 100 mg/L were between 87 and 103% of the nominal values at the start and the end of the test. The test item was stable in the test media over the test period of 72 hours. The biological results were related to the nominal concentrations of the test item.

The biological results of the study were as follows:

EC10 >100 mg/L (growth rate); 60 mg/L (yield)

EC50 >100 mg/L (growth rate); >100 mg/L (yield)

EC90 >100 mgL/L (growth rate); >100 mg/L (yield)

NOEC ≥100 mgL/L (growth rate); 46 mg/L (yield)

LOEC ≥100 mgL/L (growth rate); 100 mg/L (yield)

 

Description of key information

EC10 and EC50 were determined to be >100 mg/L, related to the growth rate.

Additional information

A test on growth inhibition to Scenedesmus subspicatus was conducted according to OECD 201. Analytical measurements revealed that the test substance concentration was >80% during the test. After 72 h of exposure, both EC10 and EC50 were determined to be >100 mg/L, related to the growth rate.