Tetraethyllead

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. Reliability assessment as per that quoted in IUCLID 4 (2000) data review

Data source

Reference

Reference Type:

publication

Title:

The LD50 value of tetraethyl lead

Author:

Schroeder, T., Avery, D.D., Cross, H.A

Year:

1971

Bibliographic source:

Experimentia 28, 425-426

Materials and methods

Principles of method if other than guideline:

16 animals assigned to 4 groups were dosed via intragastric intubation with with different levels of TEL dissolved in peanut oil. Mortality data was collected over a period of 14 days and the LC50 value determined by producing a response curve

GLP compliance:

not specified

Test type:

fixed dose procedure

Test material

Test material information

Constituent 1

Reference substance name:

Tetraethyllead

EC Number:

201-075-4

EC Name:

Tetraethyllead

Cas Number:

78-00-2

Molecular formula:

C8H20Pb

IUPAC Name:

tetraethylplumbane

Details on test material:

98% purity

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

8 males (350 - 425 grams in weight) and 8 female (150 - 250 grams in weight)
Temperature and light controlled confined room with 12 hour light cycles with no contact to sunlight or UV light

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

peanut oil

Details on oral exposure:

TEL at 7.32 mg/ml of pure peanut oil
2 ml of oil administered per animal
Single dose then observation for 14 days to collect mortality data

Doses:

4 groups dosed at 10/13/16.9/21.97 mg TEL/kg bw

No. of animals per sex per dose:

Equal number of males and females per group

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

14.18 mg/kg bw

Based on:

test mat.

95% CL:

> 12.62 - < 15.93

Mortality:

Mortalities after 14 days observed were:
1 of 4 @ 10 mg/kg
0 of 4 @ 13 mg/kg
4 of 4 @ 16.9 mg/kg
4 of 4 @ 21.97 mg/kg

Any other information on results incl. tables

All animals displayed neurological signs including lethargy, irritability and ataxia from day 2. Violent trembling and aggressiveness then followed from day 5 with convulsions and death from day 6. These signs appeared sooner in those animals receiving the higher doses

Applicant's summary and conclusion

Interpretation of results:

very toxic

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

An LD50 value of 14.18 mg/kg is reported in rats

Executive summary:

Schroeder (1972) showed the oral LD50 of TEL to be 14.18mg/kg bw. The research was based on a sample group of 16 rats in four groups or four with each group receiving a different dose level of TEL. All animals displayed neurological signs including lethargy, irritability, ataxia, violent trembling and aggressiveness. In animals dosed at the higher two dose levels death followed from day 6 and all 8 animals died by the end of the study on day 14. The LD100 was 16.9mg/kg bw