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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Link to relevant study record(s)

fish early-life stage toxicity
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
The source substance is the racemic form of the target substance d-tetramethrin. Tetramethrin does consist of 50% d- and l-form, and both do exist as cis and trans isomers. Hence, the target substance does consist of d-cis-tetramethrin, d-trans-tetramethrin, l-cis-tetramethrin and l-trans-tetramethrin, whereas the target substance only contains the first two (i.e. d-cis-tetramethrin, d-trans-tetramethrin).
Both, the source as well as the target substance, do have a cis/trans ratio of approximately 1/4 and obviously do share same molecular formula and mass, functional groups and other properties. Thus, the source substance by definition does contain ~50% of the d-form and the l-form is not expected to be significantly different with respect to its toxic properties. Acute toxicity to fish has been assessed in two acute fish toxicity tests (semi-static and flow through method) and one fish early life stage study, forming a solid foundation for assessing aquatic toxicity to fish. Accordingly, data do indicate that racemic tetramethrin and thus also d-tetramethrin is very toxic to fish and results of d-tetramethrin are not expected to significantly differ from the racemic mixture, considering that this contained the d-enantiomer as the main component.
The d-tetramethrin with a purity > 80 % does contain its corresponding l-form as an impurity in the range of < 7%, other impurities from the manufacturing process are individually below 1% (w/w) each. The source substance that has been tested was having a purity of approx. 98% as tetramethrin with a cis/trans ratio of 1/4 and a d-form/l-form ratio of ~50/50.
Source and Target substance do share identical structure and molecular weight, only differentiating by the fact that the source substance is a racemic mixture, whereas the target substance represents almost pure d-form. Thus, behaviour regarding toxicity to fish is expected not being affected significantly by stereochemistry, and being fully comparable, thus justifying using available data on fish toxicity properties on the racemic form for read-across to the d-enantiomer.
Composition comparison
D-tetramethrin (target) tetramethrin (source)
D-trans tetramethrin 70 - 80% 40 – 50%
D-cis tetramethrin 10 - 20% 7 – 11 %
L-trans tetramethrin 0 – 5% 35 – 40%
L-cis tetramethrin 0 – 2% 7 – 11%
Reason / purpose for cross-reference:
read-across source
28 d
Dose descriptor:
Effect conc.:
6.5 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: embryo hatching success, percentage of embryos that produce live, normal larvae at hatch, larval survival and larval growth
Based on mean measured concentrations, the NOEC and LOEC for the biological endpoints were determined to be 6.5 µg/L (10 µg/L nominal, highest conc. tested) and > 6.5 µg/L, respectively. The results found with racemic tetramethrin are considered representative for d-tetramethrin too.

Description of key information

To determine the Lowest-Observed-Effect Concentration (LOEC) and the No-Observed-Effect Concentration (NOEC) of tetramethrin T.G. to fathead minnow (Pimephales promelas) embryos and larvae under flow-through conditions a Early Life-Stage Toxicity Test has been conducted according to OECD guideline 210.

Based on mean measured concentrations, the NOEC and LOEC for the biological endpoints were determined to be 6.5 µg/L and > 6.5 µg/L, respectively, considered being also representative for d-tetramethrin.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
6.5 µg/L

Additional information