3,7-dimethyloctane-1,7-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24.10.2017 to 26.10.2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

GLP compliance:

yes (incl. QA statement)

Remarks:

BioChem agrar, Labor für biologische und chemische Analytik GmbH, Kupferstr. 6, 04827 Machem OT Gerichshain (Germany)

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethyloctane-1,7-diol

EC Number:

203-517-1

EC Name:

3,7-dimethyloctane-1,7-diol

Cas Number:

107-74-4

Molecular formula:

C10H22O2

IUPAC Name:

3,7-dimethyloctane-1,7-diol

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Miltitz Aromatics GmbH (Bitterfeld-Wolfen, Germany), Batch No.: BM 7/17, Lot No.: 239/17
- Expiration date of the lot/batch: 14.06.2019
- Purity test date: 14.06.2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: hydroxycitronellol was stored in the original container, at room temperature (at BioChem agrar GmbH: at ca. 20°C, dark and dry)
- Stability under test conditions: yes

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no
- Final dilution of a dissolved solid, stock liquid or gel: Due the water solubility of the test item ca. 30 g/L at 25°C), a stock solution was prepared. The desired test concentration of ca. 100 mg/L was achieved by dilution.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test item hydroxycitronellol is soluble in water (ca. 30 g/L at 25°C). A stock solution was prepared by weighing of 53 mg test item into a graduated flask and filling up to 500 mL using test medium. The test solutions were prepared directly in the test vessels.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea (Daphnia magna Straus)
- Source: Origin of the test organism: RWTH Aachen University, Institute for Environmental Research (Biology V), Worringerweg 1, 52074 Aachen Germany); origin of the animals used in the test: test animals held and bred in the test facility under standardised laboratory conditions, test animals derived from a healthy stock, not first brood progeny
- Age of parental stock (mean and range, SD): not reported
- Feeding during test: no

ACCLIMATION: not necessary

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

232 mg/L CaCO3 (test medium)

Test temperature:

19.7-20.5°C in all test vessels during the test

pH:

7.76-7.87 in all test vessels during the test

Dissolved oxygen:

8.67-8.79 mg/L in all test vessels during the test

Conductivity:

1.3 µS/cm (test medium)

Nominal and measured concentrations:

100 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass/25 mL/-/10 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): study conducted under static conditions
- Renewal rate of test solution (frequency/flow rate): not applicable (study conducted under static conditions)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 (+3 additional vessels: 1 for measuring of water parameters, 1 for analysis, 1 retain specimen)
- No. of vessels per control (replicates):4 (+3 additional vessels: 1 for measuring of water parameters, 1 for analysis, 1 retain specimen)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 1.3 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
- Light intensity: ca. 20 µE/m² x s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility

VEHICLE CONTROL PERFORMED: no (no veheicle used)

RANGE-FINDING STUDY: performance not reported

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

In the most recent study, the 24 h EC50 was determined to be 1.34 mg/L potassium dichromate. Thus, the reference item EC50 was within the expected range of toxicity (1.0-2.0 mg/L).

Reported statistics and error estimates:

Due to the test results, nstatistical calculations were performed.o

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test, the 48 h NOEC, LOEC, and EC50 values based on a nominal test concentration were determined to be >=100, >100, and >100 mg/L hydroxycitronellol, respectively.

Executive summary:

The acute toxicity of hydroxycitronellol to Daphnia magna was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test; 2004). In this limit test, 20 daphnids were exposed to a nominal concentration of 100 mg/L under static conditions for 48 h. Immobility and abnormal behaviour/symptoms of the test animals were recorded. The 48 h NOEC, LOEC, and EC50 values based on a nominal test concentration were determined to be >=100, >100, and >100 mg/L, respectively.

The study is well documented and can be classified as reliable without restrictions satisfying the guideline requirements for an acute toxicity study with invertebrates.

Results Synopsis

Test organism/age: Daphnia magna (<24 h old)

Test Type: static

48 h NOEC: >=100 mg/L (nominal)

48 h LOEC: >100 mg/L (nominal)

48 h EC50: >100 mg/L (nominal)

Endpoint(s) Effected: mobility, behaviour/symptoms