Chlorimuron ethyl

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Remarks:

Loss of typical motility

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

This study was conducted to evaluate the effects of the test substance on Spirillum volutans, through the loss of typical motility of this bacteria. Plates with one drop of each sample were prepared and observed microscopically at time zero. New plates were prepared at 5, 15 and 30 minutes’ intervals, and observed microscopically.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

ethyl 2-({[(4-chloro-6-methoxypyrimidin-2-yl)carbamoyl]amino}sulfonyl)benzoate

EC Number:

618-690-2

Cas Number:

90982-32-4

Molecular formula:

C15H15ClN4O6S

IUPAC Name:

ethyl 2-({[(4-chloro-6-methoxypyrimidin-2-yl)carbamoyl]amino}sulfonyl)benzoate

Test material form:

solid

Remarks:

White

Details on test material:

95 to >99% purity

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

other: Spirillum Volutans

Study design

Limit test:

no

Total exposure duration:

30 min

Test conditions

pH:

4.8

Nominal and measured concentrations:

100, 500 and 10000 mg/L

Reference substance (positive control):

yes

Remarks:

Mercurium chloride

Results and discussion

Effect concentrations

Key result

Duration:

30 min

Dose descriptor:

other: MEC90

Effect conc.:

>= 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Applicant's summary and conclusion

Conclusions:

MEC-90: 10000 mg/L (Spirillum volutans)

Executive summary:

This study was conducted to evaluate the effects of the test substance on Spirillum volutans, through the loss of typical motility of this bacteria.

The test substance was initially tested in 3 different nominal concentrations of 100, 500 and 10000 mg/L, with 2 replicates, being added to 0.1 mL of 24 hours culture of Spirillum volutans. Plates with one drop of each sample were prepared and observed microscopically at time zero.

New plates were prepared at 5, 15 and 30 minutes intervals, and observed microscopically. The minimal effective nominal concentration that causes loss of typical reverse movement for 90% of bacteria after 30 minutes of exposure was greater than 10000 mg/L, under the conditions of the test. The result indicates that under the conditions of the test, the concentration of 10000 mg/L of the product did not present acute toxic effects.

MEC-90: 10000 mg/L