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Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

From 11 March 1988 to 13 March 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

Refer to the Quaternary ammonium salts (QAS) category or section 13 of IUCLID for details on the category justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

A clear stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask. The test solutions were prepared by adding the appropriate volume of stock solution to dilution water to total 1,000 mL. Each solution was mixed on a magnetic stirrer for 30 seconds and then divided into 4 beakers to provide replicate exposure treatments, each containing 200 mL. The remaining 200 mL of test solution were used for 0 h water quality measurement.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Name of vehicle: Water
- Concentration of vehicle in test medium (stock solution): Stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Species: Daphnia magna
- Source: Springborn Life sciences, Inc., Wareham, Massachusetts
- Age at study initiation (mean and range, SD): ≤24 h
- Feeding during test: No

ACCLIMATION
- Acclimation period: Cultures of the test organism over many generation has been continuously maintained at the lab
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Solution of green algae and yeast suspension daily
- Feeding frequency: Daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

180 mg/L as CaCO3

Test temperature:

20-21⁰C

pH:

7.9-8.3

Dissolved oxygen:

98-99%

Nominal and measured concentrations:

0, 236, 394, 667, 1,091, 1,818 and 3,030 µg/L as nominal concentration

Details on test conditions:

The test was conducted in 250 mL glass beaker. A clear stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask. The test solution was prepared by adding the appropiate volume of stock solution to dilution water to a total volume of 1 L. Each solution was mixed on a magnectic stirrer for 30 seconds and then divided into 4 beakers to provide replicate exposure treatments, each containing 200 mL. The remaining 200 mL of test solution was used for 0 hour water quality measurement and then discarded. One set of 4 control beakers was prepared containing the same dilution water and maintained under the same conditions as the exposure concentration, but without the test subsatnce. The ambient air temperature was controlled in order to maintain test solution temperature at 20 ± 1⁰C. The test solution was not aerated. The test area was illuminated with Sylvania GRO-LUX and cool white fluorescent lights at an intensity of 70 footcandles at the solution surface.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

550 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

280 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: i.e., equivalent to 92.4 µg a.i. /L)

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

< 236 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality, behavioural and physical abnormalities

Remarks on result:

other: i.e., equivalent to 78 µg a.i. /L)

Details on results:

The 48 h EC50 and 95% confidence interval (calculated by probit analysis) was 280 (i.e. 92.4 ug a.i./L). The NOEC was <236 µg/L (<78 ug a.i./L), the lowest tested concentration.

Validity criteria fulfilled:

yes

Conclusions:

Based on the results of the read across study, the 48 h EC50 and NOEC of the test substance can also be considered to be 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.  

Executive summary:

A study was conducted to determine the acute toxicity study of the read across substance, Coco TMAC (33% active ingredient), to the Daphnia magna according to a method similar to OECD Guideline 202, in compliance with GLP. Daphnia magna (10 per dose) were exposed to the test substance at nominal concentrations of 0, 236, 394, 667, 1,091, 1,818 and 3,030 µg/L for 48 h under static conditions and mortalities were determined. The 48 h EC50and 95% confidence interval were calculated by probit analysis as 280 (250 - 300) µg/L. No analytical determination of the test substance was performed. The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 h EC50 and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively (Surprenant, 1988). Based on the results of the read across study, the 48 h EC50 and NOEC of the test substance can also be considered to be 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.  

Description of key information

Based on the results of the read across study, the 48 h EC50 and NOEC values of 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively has been considered further for hazard assessment of C12-14 TMAC.  

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.09 mg/L

Additional information

A study was conducted to determine the acute toxicity study of the read across substance, Coco TMAC (33% active ingredient), to the Daphnia magna according to a method similar to OECD Guideline 202, in compliance with GLP. Daphnia magna (10 per dose) were exposed to the test substance at nominal concentrations of 0, 236, 394, 667, 1,091, 1,818 and 3,030 µg/L for 48 h under static conditions and mortalities were determined. The 48 h EC50and 95% confidence interval were calculated by probit analysis as 280 (250 - 300) µg/L. No analytical determination of the test substance was performed. The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 h EC50 and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively (Surprenant, 1988). Based on the results of the read across study,the 48 h EC50 and NOEC of the test substance can also be considered to be 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.