Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl-and acetate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substane

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity to aquatic algae and cynobacteria for the test chemical :1.To evaluate toxicity to aquatic algae of the test chemical 2.To evaluate groeth inhibition potential of test chemical on Desmodesmus subspicatus

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate- Molecular formula: C23H26N3.C2H3O2- Molecular weight: 403.5231g/mole- Smiles : CC(=O)[O-].CC1(C2=CC=CC=C2[N+](=C1C=CC3=CC=C(C=C3)N(C)CCC#N)C)C- Inchl: 1S/C23H26N3.C2H4O2/c1-23(2)20-8-5-6-9-21(20)26(4)22(23)15-12-18-10-13-19(14-11-18)25(3)17-7-16-24;1-2(3)4/h5-6,8-15H,7,17H2,1-4H3;1H3,(H,3,4)/q+1;/p-1- Substance type: Organic- Physical state: Greenish lustourous liquid

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

2)The stock solution (100 g/L) was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

Test organisms (species):

other: 1.Chlorella sp 2. Desmodesmus subspicatus

Details on test organisms:

1.TEST ORGANISM- Common name: Chlorella sp.- Source : Thailand Institute of Scientific and Technical Research2.TEST ORGANISM- Common name: - Strain: 86.81 SAG- Source (laboratory, culture collection): Institute of botany of the ASCR- Initial biomass concentration: 5x10(3) cells /ml - Method of cultivation: No data availableACCLIMATION - No data available- Acclimation period:- Culturing media and conditions (same as test or not):- Any deformed or abnormal cells observed:

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

2) 23 ± 2°C

pH:

2) without adjustmentThe sample concentration 50 mg/l: pH=8.0 (changed to pH=7.5 during the test)Control: pH=8.1 (changed to pH= 7.7 during the test)Control + acetone: pH=8.1 (changed to pH= 7.7 during the test)

Nominal and measured concentrations:

1) 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6%2) 0, 3, 6, 12, 24 and 50 mg/L

Details on test conditions:

1) - Test vessel: 500 ml Erlenmeyer flask-fill volum fill volume: 100 ml-No. of organisms per: final concentrationof 1 £ 105 cells/mL- Light intensity and quality: cool white fluorescentlight approximately 120 µE/s/m2 or 6,000 lux2)TEST SYSTEM- Test vessel: 50/ 100 ml Glass vessel- Type (delete if not applicable): closed (with air permeable stopper)- Sample volume: 15 ml- Initial cells density: 5x10(3) cells/ml- No. of vessels per concentration (replicates): 3GROWTH MEDIUM- Standard medium used: yesOTHER TEST CONDITIONS- Adjustment of pH: No- Photoperiod: Continuous- Light intensity and quality: 6000-8000 lxEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7) for read across 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

10.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Read across 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

12.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Read across 2

Details on results:

1)Determination of algal growth by measuring live cell count,Chlorophyll a, dry weight and absorbance at 720nm

Results with reference substance (positive control):

2) Results with reference substance valid- EC50: 0.69 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate is likely to be toxic to aquatic algae in the dose range of 10.8 to 12.9 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate.The studies are as mentioned below.

1.The algal growth inhibition test was performed using as test material on Chlorella sp .

 Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .

 The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.

Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.

2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.

The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Thus, based on the above summarised studies, Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation, Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate can be classified

for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemical Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate can be classified as Aquatic Chronic category 3 as per the CLP criteria.

Description of key information

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate.The studies are as mentioned below.

1.The algal growth inhibition test was performed using as test material on Chlorella sp .

 Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .

 The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.

Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.

2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.

The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Thus, based on the above summarised studies,Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetateand it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation,Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetatecan be classified

for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemicalReaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetatecan be classified as Aquatic Chronic category 3 as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

10.8 mg/L

Additional information

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the toxicity of aquatic algae and cyanobacteria of the test chemical Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate.The studies are as mentioned below.

1.The algal growth inhibition test was performed using as test material on Chlorella sp .

 Chlorella sp were grown on 500mL Erlenmeyer flask.Algae were added into 100mL of test water containing 50, 100, 150, 200 and 250 mg/L of 73.6, 67.9, 33.3, 25.0 and 16.6% concentration of test chemical .

 The flask was placed in an ambient temperature provided air and sealed with parafilm.Flasks were continuous illuminated (cool white fluorescent light approximately 120 mE/s/m2 or 6,000 lux at water level.

Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720nm. The effect concentration of test chemical on Chlorella sp. was observed to be 10.8 mg/l.

2.Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance,according to OECD Guideline 201.

The test substance was dissolved in DMSO/ OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Thus, based on the above summarised studies,Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetateand it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 10 to 12 mg/ L. Thus, comparing this value with the criteria of CLP regulation,Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetatecan be classified

for toxicity for aquatic algae and cynobacteria .Hence,based on the data available for structually and functionally similar read across, test chemicalReaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetatecan be classified as Aquatic Chronic category 3 as per the CLP criteria.