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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material: Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino]phenyl]ethenyl]-1,3,3-trimethyl- & acetate- Molecular formula: C23H26N3.C2H3O2- Molecular weight: 403.5231g/mole- Smiles : CC(=O)[O-].CC1(C2=CC=CC=C2[N+](=C1C=CC3=CC=C(C=C3)N(C)CCC#N)C)C- Inchl: 1S/C23H26N3.C2H4O2/c1-23(2)20-8-5-6-9-21(20)26(4)22(23)15-12-18-10-13-19(14-11-18)25(3)17-7-16-24;1-2(3)4/h5-6,8-15H,7,17H2,1-4H3;1H3,(H,3,4)/q+1;/p-1- Substance type: Organic- Physical state: Greenish lustourous liquid-Batch No.: KCP/FS/83/18
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hr stirring,After stirring stock was analytically detected ,the solubility found 848.46 mg/L.Considering this value following test concentrations prepared 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source: Anytime Pet,Jaripatka, Nagpur- Length at study initiation (length definition, mean, range and SD):average 1.29 cm- Weight at study initiation (mean and range, SD): average 0.049g (average)-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.-Duration of housing: 15 days -Photoperiod: 16 hours light and 8 hours dark-Oxygen content :7.0 (mg/l)--Diet : Standard brand feed, once daily-Acclimatization: seven days -feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
155.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.2
Dissolved oxygen:
7.4 mg/L
Nominal and measured concentrations:
6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
Details on test conditions:
TEST SYSTEM - Test vessel: plastic aquaria - volume: 2 liters - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 fishes OTHER TEST CONDITIONS - Photoperiod:16 hours light and 8 hours dark
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: no mortality observed
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 50% mortality observed
Key result
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

 

8.1. Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

6.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

12.5

4

4

-

-

-

-

-

-

-

-

-

1

2

2

25

4

4

-

-

-

-

4

4

4

4

4

4

4

4

50

4

4

-

-

-

-

4

4

4

4

4

4

4

4

100

4

4

-

-

-

-

4

4

4

4

4

4

4

4

               

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

6.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

12.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS3

NS2

NS2

25

4

4

NS4

NS4

M4

M4

-

-

-

-

-

-

-

-

50

4

4

NS4

NS4

M4

M4

-

-

-

-

-

-

-

-

100

4

4

NS4

NS4

M4

M4

-

-

-

-

-

-

-

-

 

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

 

pH and oxygen content:

Nominal

Concentration

(mg/l)

Number of

fish

pH readings after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.3

7.3

7.4

7.4

7.5

7.5

7.6

7.6

6.25

4

4

7.5

7.5

7.6

7.6

7.7

7.7

7.8

7.8

12.5

4

4

7.5

7.5

7.7

7.7

7.8

7.8

7.9

7.9

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

 

Nominal

Concentration

(mg/l)

Number of

fish

Oxygen content (mg/L) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.9

7.0

6.9

6.8

6.8

6.8

6.7

6.7

6.25

4

4

6.9

6.9

6.8

6.8

6.7

6.6

6.5

6.5

12.5

4

4

6.9

6.9

6.7

6.7

6.7

6.7

6.6

6.6

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

 

 

 

 

Test temperature:

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.2

23.2

23.2

23.2

23.2

23.3

23.3

23.3

6.25

4

4

23.2

23.2

23.2

23.2

23.3

23.3

23.3

23.3

12.5

4

4

23.2

23.2

23.2

23.3

23.3

23.3

23.3

23.3

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

 

Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No 65122-06-7 on Zebra fish(Danio rerio)was found to be 12.5 mg/L.
Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino] phenyl]ethenyl]-1,3,3-trimethyl- & acetate on Zebra fish.

The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L andZebra FishDanio rerio were exposed to these concentration for 96 hours.

 

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hr stirring,After stirring stock was analytically detected ,the solubility found 848.46 mg/L.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L ,respectively and Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be 12.5 mg/l

After 96 hours of exposure to test item Reaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino] phenyl]ethenyl]-1,3,3-trimethyl- & acetate to various nominal test concentrations, LC50 was determine to be 12.5 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as "Acute aquatic 2" as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted forReaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino] phenyl]ethenyl]-1,3,3-trimethyl- & acetateon Zebra fish.

The nominal concentration selected for the experiment were6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/LandZebra FishDanio reriowere exposed to these concentration for 96 hours.

 

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hr stirring,After stirring stock was analytically detected ,the solubility found 848.46 mg/L.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment were6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L ,respectivelyand Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be 12.5 mg/l

After 96 hours of exposure to test itemReaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino] phenyl]ethenyl]-1,3,3-trimethyl- & acetateto various nominal test concentrations, LC50 was determine to be 12.5 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as "Acute aquatic 2" as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
12.5 mg/L

Additional information

Short-term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted forReaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino] phenyl]ethenyl]-1,3,3-trimethyl- & acetateon Zebra fish.

The nominal concentration selected for the experiment were6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/LandZebra FishDanio reriowere exposed to these concentration for 96 hours.

 

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hr stirring,After stirring stock was analytically detected ,the solubility found 848.46 mg/L.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment were6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L ,respectivelyand Zebra fish were exposed to this concentration for 96 hours. The lethal concentrations LC50 was found to be 12.5 mg/l

After 96 hours of exposure to test itemReaction mass of 3H-Indolium, 2-[2-[4-[(2-cyanoethyl)methylamino] phenyl]ethenyl]-1,3,3-trimethyl- & acetateto various nominal test concentrations, LC50 was determine to be 12.5 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as "Acute aquatic 2" as per the CLP classification criteria.