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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to aquatic invertebrates:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

2. Based on the mobility of test organism daphnia magna by providing the exposure period of 48 hrs, the EC50 was determine to be < 0.1

Based on both studies it was concluded that the reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate was concluded as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria:

1. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively.

2. Based on the effect on growth rate and biomass of the test organism Pseudokirchneriella subcapitata, the 72hr ErC50and EbC50 values of test chemical was determined as 0.83 and 0.44 mg/L, respectively.

Based on both studies it was concluded that the reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Additional information

Short term toxicity to aquatic invertebrates:

Data available for the structurally and functional similar read across chemicals has been reviewed to determine the toxicity of the test chemical Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate on the growth of aquatic invertebrates. The studies are as mentioned below:

 

In the first study from experimental report determination of the inhibition of the mobility of daphnids was carried out with the substance according to OECD Guideline 202.The stock solution 20 mg/L was prepared by dissolving dark violet liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0.006, 0.012, 0.025, 0.050, 0.100 mg/l. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.037 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 as per the CLP criteria.

 

Similarly the second study was conducted to evaluate the influence of test chemical on the mobility of Daphnia magna. Under the conditions of the study test chemical did induce acute immobilisation of Daphnia magna in all the nominal concentrations tested i.e 0.1, 1.0, 10 and 100 mg/l within 24 hours of exposure. Therefore, the 48h-EC50 value was determine to be <0.1 mg /l. And the chemical was concluded as toxic and classified as aquatic chronic 1.

 

Based on both studies it was concluded that the chemical Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate was concluded as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate. The studies are as mentioned below:

 

In the first study from authoritative database short term toxicity to green algae study was carried out for 72 hrs. The study was based on the effects of the test compound on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

 

Similarly second study was from secondary sources in which short term toxicity to Pseudokirchneriella subcapitata (algae) study was carried out for 72 hrs. The study was performed according to OECD Guideline 201 (Alga, Growth Inhibition Test). The study was based on the effects of the test compound on Pseudokirchneriella subcapitata in a static fresh water system. Pseudokirchneriella subcapitata was used as test organism. Analytical monitoring was done in which the NPOC (non-purgeable organic carbon) analysis was performed. Cultured algae were added to the WAF (Water – Accommodated Fraction) and Potassium dichromate was used as positive control in the study. Based on the effect on growth rate and biomass of the test organism Pseudokirchneriella subcapitata, the 72hr-ErC50and EbC50 values of test chemical was determined as 0.83 and 0.44mg/L, respectively. Thus, based on EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be classified as aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

Based on both studies it was concluded that the chemical Reaction mass of 3H-​Indolium, 2-​[2-​[4-​(dimethylamino)​phenyl]​ethenyl]​-​1,​3,​3-​trimethyl-​ & acetate was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.