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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jan 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Version / remarks:
1986
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Chromate(2-), [2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)][2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-5-sulfobenzoato(3-)]-, lithium sodium
EC Number:
288-678-6
EC Name:
Chromate(2-), [2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)][2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-5-sulfobenzoato(3-)]-, lithium sodium
Cas Number:
85865-79-8
Molecular formula:
C34H23CrLiN8NaO9S
IUPAC Name:
Chromate(2-), [2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)][2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-5-sulfobenzoato(3-)]-, lithium sodium
Test material form:
solid: particulate/powder
Details on test material:
Acid Yellow 232

Test animals

Species:
rabbit
Strain:
New Zealand White
Remarks:
HC:NZW
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Interfauna UK ltd.
- Housing: individual, Cage type III-high
- Weight at study initiation: 3.0 - 3.4 kg
- Diet (e.g. ad libitum): "ssniff K4" ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period: at least 14 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 +/- 2°C
- Humidity (%): 45 - 65%
- Photoperiod (hrs dark / hrs light): 12h/12h

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
water
Remarks:
to form a paste
Controls:
yes, concurrent no treatment
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 500 mg test material mixed with water to have a thicker paste
Duration of treatment / exposure:
4 h exposure
Observation period:
14 days
Number of animals:
3
Details on study design:
TEST SITE
- Area of exposure: 6 x 6cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with Water
- Time after start of exposure: 4h

OBSERVATION TIME POINTS
(indicate if minutes, hours or days) 1, 24, 48 and 72h and 7 and 14 days

SCORING SYSTEM:
Draize-Grade

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
4
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
mean
Time point:
24/48/72 h
Score:
0
Max. score:
4
Remarks on result:
no indication of irritation

Any other information on results incl. tables

Animal No Endpoint  Observation time 
24h 48h 72h
I erythema 0 0 0
edema 0 0 0
II erythema 0 0 0
edema 0 0 0
III erythema 0 0 0
edema 0 0 0

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
No signs of irritation related to the substance were observed.