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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
30 Nov - 02 Dec 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
EC Number:
255-527-0
EC Name:
Sodium bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)
Cas Number:
41741-86-0
Molecular formula:
C34H24CrN8O6.Na
IUPAC Name:
Sodium;chromium(3+);2-[(3-methyl-5-oxido-1-phenylpyrazol-4-yl)diazenyl]benzoate
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: control, 0.39, 6.25 and 100 mg/L
- Sampling method: directly taken from test vessel at 0 and 48 h

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stirring at 20°C for 20 h in M4 medium, 100 mg/L final concentration of eluate
- Eluate: diluted further
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): yes, removed by centrifugation for 20 min

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Age of parental stock (mean and range, SD): 2 - 4 wk
- Feeding during test: none
- Age at study initiation: 2- 24 h

ACCLIMATION
- Acclimation period: no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.36 mmol/L
Test temperature:
20.4 - 20.8°C
pH:
8.0 - 8.2
Dissolved oxygen:
8.4 - 8.8 mg/L
Conductivity:
593 µS/cm
Nominal and measured concentrations:
Nominal: 0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L
Measured: maximum 13% recovery in all measured concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel: test tubes
- Material, size, headspace, fill volume: glass, 20 mL, flat bottom
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/mL


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h dark
- Light intensity: 2 - 7 µE/(m² x s)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 0, 24 and 48 h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.56 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI: 1.88 - 3.51 mg/L
Details on results:
Mortality of control: 5%
Results with reference substance (positive control):
24-h EC50: 1.19 mg/L (normal sensitivity response)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 to Daphnia magna was determined at 2.56 mg/L (mean meaured). Due to the low recovery (max. 13%) of the test item, no nominal concentration value was useful.