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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
unknown
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
publication
Title:
Frameshift Mutagenicity of Certain Naturally Occurring Phenolic Compounds in the 'Salmo11ella/Microsome' Test: Activation of Anthraquinone and Flavonol Glycosides by Gut Bacterial Enzymes
Author:
JOSEPH P. BROWN, PAUL S. DIETRICH and RONALD J. BROWN
Year:
1977
Bibliographic source:
Biochemical Society Transactions, 5(5), 1977, pp. 1489-1492

Materials and methods

Principles of method if other than guideline:
Study conducted according to principles of AMES test as described in Ames, B. N., Mccann, J. & Yamasaki, E. (1975) Mutat, Res. 31, 347-364.
Manipulation of cultures, preparation of crude microsomal fraction (S9) from Aroclor 1254-induced rats and criteria used in scoring results were was conducted according to Brown, J.P. & Brown, R. J. (1976) Murat, Res. 40, 203-224.
GLP compliance:
no
Remarks:
Study conducted prior to implementation of GLP
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dichloro-5,8-dihydroxyanthraquinone
EC Number:
220-599-4
EC Name:
1,4-dichloro-5,8-dihydroxyanthraquinone
Cas Number:
2832-30-6
Molecular formula:
C14H6Cl2O4
IUPAC Name:
1,4-dichloro-5,8-dihydroxy-9,10-dihydroanthracene-9,10-dione

Method

Target gene:
Histidine locus
Species / strain
Species / strain / cell type:
S. typhimurium, other: TA1535, TA1537, TA1538, TA98, TA100
Metabolic activation:
with and without
Metabolic activation system:
liver microsomal S9 from Aroclor 1254-induced rats

Results and discussion

Test resultsopen allclose all
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Key result
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified

Applicant's summary and conclusion

Conclusions:
5,8-Dichlorchinizarin was evaluated in the bacterial reverse mutation assay (Ames test) using Salmonella typhimurium tester strains TA1535, TA1537, TA1538, TA98 and TA100 in the presence and absence of rat liver S9 mix. Under the conditions of the study, the test substance was negative for mutagenic potential.
Executive summary:

In a reverse gene mutation assay in bacteria according to Ames et al. (adopted 1975), Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98 and TA100 were exposed to 5,8-Dichlorchinizarin in concentrations of 10, 50, 100 and 500µg/plate in the presence and absence of mammalian metabolic activation (rat liver S9 mix).

 

Revertants/nmol, i.e. the increase in numbers of revertants/plate per nmol increase in the quantity of test agent over the linear portion of the dose-response curve was <0.01 in the test groups with and without metabolic activation. 

Accordingly, no substantial increase in revertant colony numbers of any of the five tester strains was observed following treatment with 5,8-Dichlorchinizarin at any concentration level, neither in the presence nor absence of metabolic activation (S9).

 

There was no evidence of induced mutant colonies over background.

 

Under the conditions of the study, the test substance was negative for mutagenic potential.