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Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Specific details on test material used for the study:
- SMILES: O(C(CCC1C2)(C2)C)C1(C)C
- MOL FOR: C10 H18 O1
- MOL WT: 154.25
- log Kow : 3.133 (EPISuite, kowwin v1.68 estimate)
- Water solubility : 3500 mg/L (PhysProp DB exp value)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
8.805 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 8.805 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 8.805 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (3.133) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
1.273 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 1.273 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 1.273 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (4.269) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
1.123 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 1.123 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 1.123 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (4.347) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Specific details on test material used for the study:
- SMILES: C=C1C(C2)C2(C(C)C)CC1
- MOL FOR: C10 H16
- MOL WT: 136.24
- log Kow : 4.689 (EPISuite, kowwin v1.68 estimate)
- Water solubility : 2.494 mg/L (EPISuite, WSkowwin v1.43 estimate)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
0.651 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 0.651 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 0.651 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (4.689) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Specific details on test material used for the study:
- SMILES: OC(C(CCC(=C1)C)C1)(C)C
- MOL FOR: C10 H18 O1
- MOL WT: 154.25
- log Kow : 3.332 (EPISuite, kowwin v1.68 estimate)
- Water solubility : 1980 mg/L (PhysProp DB exp value)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
6.416 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 6.416 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 6.416 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (3.332) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Specific details on test material used for the study:
- SMILES: C(=CCC(=C1)C)(C1)C(C)C
- MOL FOR: C10 H16
- MOL WT: 136.24
- log Kow : 4.749 (EPISuite, kowwin v1.68 estimate)
- Water solubility : 8.68 mg/L (PhysProp DB exp value)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
0.592 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 0.592 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 0.592 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (4.749) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Specific details on test material used for the study:
- SMILES: C(C=C)(=C)CCC=C(C)C
- MOL FOR: C10 H16
- MOL WT: 136.24
- log Kow : 4.877 (EPISuite, kowwin v1.68 estimate)
- Water solubility : 5.6 mg/L (PhysProp DB exp value)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
0.483 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 0.483 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 0.483 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (4.877) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Study period:
2017-10-10
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction
Qualifier:
according to guideline
Guideline:
other: REACH guidance on QSARs R.6, May 2008
Deviations:
no
Principles of method if other than guideline:
See attached QPRF.
GLP compliance:
no
Remarks:
(not relevant)
Specific details on test material used for the study:
- SMILES: OC(CCC(=C1)C)(C1)C(C)C
- MOL FOR: C10 H18 O1
- MOL WT: 154.25
- log Kow : 3.332 (EPISuite, kowwin v1.68 estimate)
- Water solubility : 386.6 mg/L (EPISuite WSKowwin v1.43 Estimate)
Details on sampling:
Not applicable
Details on test solutions:
Not applicable
Details on test organisms:
None
Total exposure duration:
96 h
Post exposure observation period:
Not applicable
Hardness:
Not applicable
Test temperature:
Not applicable
pH:
Not applicable
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Not applicable
Details on test conditions:
Not applicable
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
6.416 mg/L
Remarks on result:
other: ECOSAR Class: Neutral Organic SAR (Baseline toxicity)
Details on results:
Validity of model:
1. Defined Endpoint: Toxicity to aquatic algae.
2. Unambigous algorithm: Linear regression QSAR; Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253. To convert the EC50 from mmol/L to mg/L, multiply by the molecular weight.
3. Applicability domain: applicable to chemicals with log Kow less than 6.4 and molecular weight less than 1000 g/mol. The model can be suitable for chemicals class of neutral organic.
4. Statistical characteristics: N = 41+9 and the coefficient of determination R² = 0.6782.
5. Mechanistic interpretation: related to the partitioning of the substance from water into the organisms lipid phase.

Adequacy of prediction: the substance falls within the applicability domain described above and therefore the predicted value can be considered reliable.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
N = 41+9 and the coefficient of determination R² = 0.6782.

None

Validity criteria fulfilled:
yes
Conclusions:
The substance was predicted to have an EC50 of 6.416 mg/L to green algae after 96h of exposure.
Executive summary:

Toxicity to green algae was estimated using the ECOSAR v1.11 QSAR model available from the U.S. EPA. The estimation 96h-EC50 was 6.416 mg/L (neutral organic SAR, baseline toxicity), calculated from an estimated log Kow value (3.332) and a linear regression:

Log 96h-EC50 (mmol/L) = -0.6922 (log Kow) + 0.9253.

The substance falls within the applicability domain and therefore the predicted value can be considered reliable.

Description of key information

Constituent approach, additivity formula:

96h-EC50 for freshwater algae = 2.01 mg/L for Ravintsara oil.

Key value for chemical safety assessment

EC50 for freshwater algae:
2.01 mg/L

Additional information

Based on a weight of evidence approach, the acute toxicity of the substance to aquatic algae can be estimated using adequate toxicity data of major constituents. Constituents above 1% in the composition were considered, representing 93.96% of the mixture. The individual toxicity of these constituent has been predicted using ECOSAR v1.11 from EPISUITE tool. All the constituents fall within the applicability domain of the model and the predictions were considered reliable. Moreover, all the constituents taken into account in the assessment have the same MoA (Neutral organics, baseline toxicity).

The estimation of the toxicity of the substance was based on the individual toxicities of each constituent above 1% using typical percentages of these compounds. These data are summarized in the table below:

Constituents

Typical value in the mixture*

Freshwater algae

96h-EC50, mg/L

Cineol 1,8

55.75%

8.805

Sabinene

16.58%

0.651

Terpineol α

7.23%

6.416

Pinene α

 5.38% 1.273
 Pinene beta  3.72% 1.123
 Terpinen 4 ol  2.26%  6.416
 Myrcene  1.82%  0.483
 Gamma-terpinene  1.22%  0.592

* Typical % taken from the certificate of analysis related to the batch no B790145, provided by the supplier of the substance.

The acute toxicity of the susbtance to algae was estimated using the following additivity formula, as recommended in the Regulation (EC) No 1272/2008 (CLP):

∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)

Where,

Ci = concentration of component i (weight percentage);

L(E)C50i = LC50 or EC50 for component i, in mg/L;

L(E)C50m = L(E)C50 of the part of the mixture with test data;

 

Based on data presented above, the 96h-EC50m value for the substance was estimated to be 2.01 mg/L for aquatic algae for a typical mixture.