Reaction product of tetrahydro-2,5-dimethoxy furan, ethanol and water

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Fresenius

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At ambient temperature, protected from light
- Solubility and stability of the test substance in the solvent/vehicle: Amber liquid, indefinitely soluble
at 20°C

Analytical monitoring:

yes

Details on sampling:

The level of TOC measured in freshly prepared test media samples and aged (after 3 days) test media samples, were compared against the carbon content of the test item measured

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: clone 5
- Source: Dr. M. Bergtold, BASF, D-67117 Limburgerhof; the strain originally derived from the Institut National de Recherche Chimique Appliquee, France in 1978
- Age of parental stock: <24 h
- Feeding during test: no

ACCLIMATION
- Type and amount of food: Desmodesmus subspicatus- and/or Chlorella-cells

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19.4 - 20.1 °C

pH:

8.60 - 9.19

Dissolved oxygen:

6.6 - 8.7 mg O2/L

Nominal and measured concentrations:

The test was performed using concentration steps 0.5 mg/1000 mL, 1.1 mg/1000 mL, 2.42 mg/1000 mL, 5.32 mg/1000 mL, 11.71 mg/1000 mL, and 25.77 mg/1000 mL dilution water.
Recoveries of >64 % of the initial values measured at time t0 were obtained in the treatments at concentrations around the EC50 after 48 hr of incubation. Most measured concentrations of the treatments after 48 hr deviated more than 20% from the nominal values. Based on this, the reported effect concentrations should refer to mean measured concentrations (geometric mean) of the measured concentrations.

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: medium M4 acc. Elendt

OTHER TEST CONDITIONS
- Photoperiod: 16 hr of light and 8 hr of darkness

EFFECT PARAMETERS MEASURED:
After 24 hr and 48 hr the number of immobilised daphnia at each concentration of the test item is recorded

Reference substance (positive control):

yes

Remarks:

The effect of potassium dichromate towards different animals of this breed was tested prior to beginning of the test. Within the analytical part, the test item served as a reference item.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

12.96 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24 hr) was 0.95 mg/L potassium dichromate

 Concentrations of the Test Item during Incubation:

 

Nominal Conc. mg/L]	mg/L TOC net t0	mg/L TOC net t72hr	% of t0	Mean^1)Concentration TOC [mg/L]	Calculated mean^1)Concentration of the Test Item [mg/L]	% Recovery of the Nominal Concentrations
0	0	0	-	0	0	-
0.5	0.10	0.02	22.3	0.05	0.26	52.5
1.1	0.21	n.d.	n.d.	n.d.	n.d.	n.d.
2.42	0.41	0.14	35.0	0.24	1.35	55.8
5.32	0.98	0.45	46.3	0.67	3.69	69.3
11.71	2.11	1.35	64.2	1.69	9.33	79.7
25.77	4.18	3.66	87.7	3.91	21.61	83.9

¹⁾Geometric mean concentration

 

Results:

	Basis: Measured Concentrations [mg/L]
	t24hr	t48hr
NOEC	1.47	1.47
LOEC	3.79	3.79
EC10	5.28	3.86
EC20	8.46	5.85
EC50	20.88	12.96

n.d. = not determined for mathematical reasons.

Validity criteria fulfilled:

yes

Conclusions:

In the test on acute toxicity to Daphnia, the EC50 was determined to be 12.96 mg/L (based on measured concentrations).

Executive summary:

„Reaction Product from Tetrahydro-2,5-dimethoxyfurane, Ethanol and Water“ was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to investigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. Under the conditions used for the test, there was a immobilisation of the daphnia at concentrations >2.4 mg/L. As a conclusion of the analytical part of this study, it can be stated that the concentrations of „Reaction Product from Tetrahydro-2,5-dimethoxyfurane, Ethanol and Water“ remained sufficiently stable during incubation of 48hr only at the highest concentration step of 25.77 mg/L.At lower concentrations minor TOC concentrations were found. As a result of the supporting analyse it can be stated that the results of the biological part should be based on the measured concentrations applied because the recovery of the nominal concentrations was <80%.

Description of key information

„Reaction Product from Tetrahydro-2,5-dimethoxyfurane, Ethanol and Water“ was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to investigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. Under the conditions used for the test, there was a immobilisation of the daphnia at concentrations >2.4 mg/L.

As a conclusion of the analytical part of this study, it can be stated that the concentrations of „Reaction Product from Tetrahydro-2,5-dimethoxyfurane, Ethanol and Water“ remained sufficiently stable during incubation of 48hr only at the highest concentration step of 25.77 mg/L. At lower concentrations minor TOC concentrations were found. As a result of the supporting analyse it can be stated that the results of the biological part should be based on the measured concentrations applied because the recovery of the nominal concentrations was <80%.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

12.96 mg/L