Sulfuric acid, mono-C12-14-alkyl esters, magnesium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 - 26 Jan 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: no data
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data

ACCLIMATION
- Acclimation period: 96h under flow-through conditions

FEEDING DURING TEST
- none

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

22.0 - 22.5 °C

pH:

7.0 - 7.7

Dissolved oxygen:

10 - 100%
Oxygen is limiting on the highest concentration (75 mg product/L). However, at the other concentrations oxygen was not limiting. Since for the LD50 calculation the highest concentration is not relevant, the oxygen depletion at this concentration does not devaluate the test.

Nominal and measured concentrations:

0, 10, 15, 20, 30, 45, 60, 75 mg/L of product (nominal concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: 3 L water tanks filled with 2 L of test water
- Type of flow-through (e.g. peristaltic or proportional diluter): no data
- Renewal rate of test solution (frequency/flow rate): 4 times the test volume (2 L) per day
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: copper and chlorine free drinking water


OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16 hours light/ 8 hours dark


EFFECT PARAMETERS MEASURED:
- Mortality recorded at 24-hour interval

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.9 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: for tested product

Details on results:

- Behavioural abnormalities: no
- Abnormal responses: no

Sublethal observations / clinical signs:

Table: Fish mortality [%]

Concentration of product [mg/L]	0 h	6 h	24 h	48 h	72 h	96 h
0	0	0	0	0	0	0
10	0	0	0	0	0	0
15	0	0	0	0	0	0
20	0	0	0	10	20	20
30	0	0	90	90	90	90
45	0	0	100	100	100	100
60	0	100	100	100	100	100
75	0	100	100	100	100	100

Description of key information

96h LC50 = 7.9 mg a.i./L (Danio rerio, nominal concentration, OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

7.9 mg/L

Additional information

One short term fish toxicity test is available for C12-14AS Mg (CAS 90583-23-6). The GLP non-limit test (BASF, 2002) was performed with D. rerio according to OECD 203 guideline under static conditions. The fish were exposed to five test concentrations up to 75 mga.i./L. The 96-h LC50 value was 7.9 mg a.i./L (nominal concentration).