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EC number: 251-132-2 | CAS number: 32634-68-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity study of aquatic invertebrate for the test chemical :
1.To evaluate toxicity efficiency of test material on Daphnia magna
2.To evaluate short term toxicity of test material on aquatic invertebrates - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: 2,3-bis[(4-methylbenzoyl)oxy]succinic acid
- IUPAC name: (+)-Di-p-toluoyl-D-tartaric Acid
- Molecular formula: C20H18O8
- Molecular weight: 386.3542 g/mole
- Smiles : Cc1ccc(cc1)C(=O)O[C@@H]([C@H](OC(=O)c2ccc(C)cc2)C(=O)O)C(=O)O
- Inchl: 1S/C20H18O8/c1-11-3-7-13(8-4-11)19(25)27-15(17(21)22)16(18(23)24)28-20(26)14-9-5-12(2)6-10-14/h3-10,15-16H,1-2H3,(H,21,22) (H,23,24)/t15-,16-/m0/s1
- Substance type: Organic
- Physical state: Solid powder (white) - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 1.- Method of breeding: Daphnia magna Straus, of a single clone was maintained in a modified manure-soil medium
-Age: <=8 h
-This was made up in batches of five to ten liters in the following
proportions : 5 g. air dried horse manure, 25 g. dried sandy muck, and 1
liter tap water. After it had stood for two days the resulting infusion
was strained through silk bolting cloth. The filtrate was allowed to
stand until the turbidity disappeared, after which it was used.
- Food type: Yeast
- Amount: 250 mg
-Frequency: every alternate day - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 15 min., 30 min., 1 hr., 2 hr., 8 hr., 16 hr., and 32 hr.
- Test temperature:
- 25° C.
- Details on test conditions:
- 1.- Test vessel: wide-mouth bottles
- fill volume: 100 ml
- No. of organisms per vessel: 10 - Reference substance (positive control):
- not specified
- Duration:
- 16 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 135 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 374.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Immobility was determined directly by placing the rack of bottles containing the animals in front of a lighted translucent screen.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid is likely to be toxic to aquatic invertebrate in the dose concentration of 135 - 374.2 mg/l
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634 -68 -7).The studies are as mentioned below:
1. The test material was used to evaluate the short term toxicity test on Daphnia magna Straus .A stock of individually reared daphnids, Daphnia magna Straus, of a single clone was maintained in a modified manure-soil medium .10 daphnids of age 8 hrs were further tested . Immobility was determined directly by placing the rack of bottles containing the animals in front of a lighted
translucent screen.
No observed effect concentration (NOEC) of test material on Daphnia magna Straus was observed to be 135 mg/l.
2. Short term toxicity of test material was evaluated on daphnia magna for 48 h.The effect concentration of test material on Daphnia magna was observed to be 374.2 mg/l.
Thus, based on the above summarised studies, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 135 - 374.2 mg/L. Thus, comparing this value with the criteria of CLP regulation, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid cannot be classified for short term toxicity for aquatic invertebrate .Hence,based on the data available for the functionally similar read across, test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid is not likely to be toxic atleast in the dose range of 135 mg/L .
Reference
Description of key information
Short term toxicity to aquatic invertebrate:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634 -68 -7).The studies are as mentioned below:
1. The test material was used to evaluate the short term toxicity test on Daphnia magna Straus .A stock of individually reared daphnids, Daphnia magna Straus, of a single clone was maintained in a modified manure-soil medium .10 daphnids of age 8 hrs were further tested . Immobility was determined directly by placing the rack of bottles containing the animals in front of a lighted
translucent screen.
No observed effect concentration (NOEC) of test material on Daphnia magna Straus was observed to be 135 mg/l.
2.Short term toxicity of test material was evaluated on daphnia magna for 48 h.The effect concentration of test material on Daphnia magna was observed to be 374.2 mg/l.
Thus, based on the above summarised studies, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 135 - 374.2 mg/L. Thus, comparing this value with the criteria of CLP regulation, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid cannot be classified for short term toxicity for aquatic invertebrate .Hence,based on the data available for the functionally similar read across, test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid is not likely to be toxic atleast in the dose range of 135 mg/L .
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 374.2 mg/L
Additional information
Short term toxicity to aquatic invertebrate:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate of the test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634 -68 -7).The studies are as mentioned below:
1. The test material was used to evaluate the short term toxicity test on Daphnia magna Straus .A stock of individually reared daphnids, Daphnia magna Straus, of a single clone was maintained in a modified manure-soil medium .10 daphnids of age 8 hrs were further tested . Immobility was determined directly by placing the rack of bottles containing the animals in front of a lighted
translucent screen.
No observed effect concentration (NOEC) of test material on Daphnia magna Straus was observed to be 135 mg/l.
2.Short term toxicity of test material was evaluated on daphnia magna for 48 h.The effect concentration of test material on Daphnia magna was observed to be 374.2 mg/l.
Thus, based on the above summarised studies, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid and it’s functionally similar read across substance, it can be concluded that effect concetration value is in the range of 135 - 374.2 mg/L. Thus, comparing this value with the criteria of CLP regulation, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid cannot be classified for short term toxicity for aquatic invertebrate .Hence,based on the data available for the functionally similar read across, test chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid is not likely to be toxic atleast in the dose range of 135 mg/L .
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