1-Propanaminium, 3,3',3''-[phosphinylidynetris(oxy)]tris[N-(3-aminopropyl)-2-hydroxy-N,N-dimethyl-, N,N',N''-tri-C6-18 acyl derivs. trichlorides

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 days

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Guideline study to GLP, 2016

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Specific details on test material used for the study:

COCAMIDOPROPYL PG - DIMONIUM CHLORIDE PHOSPHATELOT # 50113A16
Date Received: 07 Mar 16;
Physical Description: Clear liquid
Storage Conditions: Room temperature
Purity: 41% active ingredient

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Initial conc.:

ca. 0.8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Two replicates removed on days 7, 14, 21 and 28, with controls removed day 0
Two replicates serving as blank controlTwo replicates service as positive control (aniline)Toxicity control was run until Day 14 to confirm activity taking place

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 30

Sampling time:

7 d

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 48

Sampling time:

14 d

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 98

Sampling time:

21 d

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 73

Sampling time:

28 d

Details on results:

Two replicates were taken on each sampling time except the final day (Day 28) with the percentage biodegradation based on a meanThe Oxygen figures for Day 21 were 5.32 and 6.80 and within an expected range if following trends from Day 0 - Day 14The Day 28 Oxygen figures ranged from 5.22 - 6.73 (excluding outliers), but the mean of 9 bottles resulted in a calculated biodegradation of 73%Based on the Day 7 and Day 21 figures, > 60% biodegradation was apparent in the 14 Day window.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Based on the Day 7 and Day 21 figures, > 60% biodegradation was apparent in a 14 Day window. The substance is considered biodegradable, although the 10-day criteria was not possible to prove. The final conclusion is backed up by examination of other similar chemical substances.

Executive summary:

Despite using a closed bottle test at low concentrations of test material, a good level of biodegradation was acheived with over 90% recorded on Day 21 (but mean measurements falling back to 73% by Day 28). The low number of sample points and low number of replicates means that it is not possible to make an accurate assessment of the 10 Day window needed for CLP Classification, but if a curve is fitted to the time points 0 - 21 Days, it is conisdered very likely that the 10 Day window was acheived.

Description of key information

Despite using a closed bottle test at low concentrations of test material, a good level of biodegradation was acheived with over 90% recorded on Day 21 (but mean measurements falling back to 73% by Day 28). The low number of sample points and low number of replicates means that it is not possible to make an accurate assessment of the 10 Day window needed for CLP Classification, but if a curve is fitted to the time points 0 - 21 Days, it is conisdered very likely that the 10 Day window was acheived.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable