Trimethyl(octadecyloxy)silane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Trimethyl(octadecyloxy)silane

EC Number:

242-554-8

EC Name:

Trimethyl(octadecyloxy)silane

Cas Number:

18748-98-6

Molecular formula:

C21H46OSi

IUPAC Name:

trimethyl(octadecyloxy)silane

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
Nominal water parameters:
Total hardness: 40- 180 mg CaC03/L
pH-value: 6.0 - 8.5
The water is analysed once a year acc. to German tap water regulation.

Test concentration, Stock solution, Dispersion Treatment: A saturated solution was prepared. 200 mg were applied to 2 L test medium and shaken with 20 rpm for 24 h (Rotaling Shaker 3040, GFL). Thereafter the suspension was treated in a centrifuge (20 min, 3000 rpm). The clear solutionwas used in the test.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Danio rerio (zebrafish), Gnathostoma, Pisces, Osteichthyes, Teleostei, Clupeiformes, Cyprinidae
Origin: AQUARIUM AM AEGI, Feldstraße 7, 30159 Hannover, Germany. All fish used in the lest originated from the same delivery of the supplier.

Holding: Holding was performed at the test facility at 23 ± 2°C and diffuse light (0.1-10 µmol/m^2 s, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value.
Only zebrafish with at least 12 days of acclimatisation and mortality < 5 % within the last 7 days before the study starts were used in the test. No disease treatments were administered throughout holding and testing.

Feeding: Food was provided 3 Iimes per week. The amount of food was 4 % of the fish body weight per feeding day. The test fish were not fed until 24 h before the test started.

Number of test fish: 7 zebrafish were used per lest concentration and control

Loading, length of fish: Fish density in the tanks was less than 1 g fish per Iiter test solution. Average body length at the test start: 2.90 cm. Average body weight at the test start: 0.27 g

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

58 mg/L as CaCO3

Test temperature:

21-21.9°C

pH:

6.71-7.33

Dissolved oxygen:

85-100% saturation

Nominal and measured concentrations:

0 + 1.3mg/l (corrected DOC saturated solution)

Details on test conditions:

Test vessel: Glass-aquaria loosely covered by glass tops were used.
Test volume: The test volume was 2 L per vessel
Aeration: Gentle aeration was provided
Dilution water: Same as for holding
Replicates: One replicate perlest concentration and control
lntroduction of fish: Fish were added to test chambers within 30 min after centrifugation of the lest item solution. Fish were introduced randomly to individual replicates.
Feeding of lest fish: The fish were not fed during the test
Light intensity: 0.1- 10 µmol/m^2s
Photoperiod: A natural photoperiod was provided du ring the lest.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

other: DOC

Basis for effect:

mortality (fish)

Remarks on result:

other: saturated solution

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

other: DOC

Basis for effect:

mortality (fish)

Remarks on result:

other: saturated solution

Details on results:

No mortality and no sub-lethal effects occured.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an OECD203 limit test no toxicity to fish (Danio rerio) could be determined up to the concetration of the saturated solution (1.3 mg/L (corrected DOC) (96h)).

Executive summary:

In an OECD203 limit test no toxicity to fish (Danio rerio) could be determined up to the concetration of the saturated solution (1.3 mg/L (corrected DOC) (96h)).