Reaction mass of Fatty acids, montan-wax and Fatty acids, montan-wax, ethylene esters and Fatty acids, montan wax, mixed esters with fatty acids C16-18 and ethylene glycol and Fatty acids, montan-wax, stearyl esters and Montan wax

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-05-05 to 2017-05-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

European Commission Regulation (EC) No. 440/2008 and No. 260/2014

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

Adopted: 27.07.95

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Specific details on test material used for the study:

Test item: WARADUR LG
Batch No.: W00004534
Expiry date: April 11, 2018
Certificate: Certificate of analysis No. 28657 dated April 23, 2013

Water solubility:

< 1 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

171 mg/L

Incubation duration:

72 h

Temp.:

20 °C

pH:

6.3

Remarks on result:

other: After specified incubation duration at 30°C, +24 h at 20°C before measurement.

Water solubility:

< 1 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

168 mg/L

Incubation duration:

48 h

Temp.:

20 °C

pH:

6.3

Remarks on result:

other: After specified incubation duration at 30°C, +24 h at 20°C before measurement.

Water solubility:

< 1 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

174 mg/L

Incubation duration:

24 h

Temp.:

20 °C

pH:

6.3

Remarks on result:

other: After specified incubation duration at 30°C, +24 h at 20°C before measurement.

Key result

Water solubility:

0.55 mg/L

Conc. based on:

other: TOC (total organic carbon) analysis

Remarks:

due to oxygen being part of the substance, the value based on MW will be somewhat higher

Loading of aqueous phase:

170 mg/L

Temp.:

20 °C

pH:

6.3

Remarks on result:

other: determined from peak analysis below LOQ given for sample "A", most probably incubation time 24 h

Details on results:

The lowest standard analyzed for calculation of the calibration curve was 1.515 mg/L. The calibration curve was highly linear and of excellent fit (see plot attached). Therefore, extrapolation to lower calibration range seems to be acceptable. The reasoning to do so is because a "lower than value" is of no worth for assessment of enviromental fate.
Attached to the report is the evaluation of one measurement ("sample A") for Waradur LG. If samples were assigned numbering according to inccreasing incubation time, 24 h incubation at 30°C followed by 24 hours equilibration at 20°C would characterize this sample. Three replicate measurements were performed, distinct peaks were obtained, and SD (concentration) was low (0.00914 mg/L). The determined mean concentration is given as 0.5465 mg/L (single rounded values: 0.5530 mg/L; 0.5237 mg/L; 0.5401 mg/L).
Because this concentration refers to TOC analysis, due to oxygen being part of the substance, the value based on MW will be somewhat higher.

Preliminary test

Three preliminary tests were carried out with specified amounts of the test item and 100 mL and 500 mL demineralized water, respectively. After each addition of an amount of water, the mixture was stirred for at least 24 hours, and visually checked for any undissolved particles.

Results of the preliminary test:

Amount of test item / mg	Total volume of demineralized water / mL	Appearance of mixture
9.5	100	not dissolved
9.1	500	not dissolved
4.2	500	not dissolved

The preliminary test showed that the water solubility of the test item is < 10 mg/L. As the test item was a mixture of different constituents the water solubility was determined by the flask method (instead of the column elution method) in the main test.

Flask method

The flask method was performed as described above. The data for the evaluation are given in the table below. After the equilibration time precipitate was observed in the flasks of the experiments excluding the blank experiment. The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given in the following table:

Water solubility experiments of the test item:

Experiment	24 h experiment	48 h experiment	72 h experiment	Blank experiment (72 h)
Amount of test item / mg	17.4	16.8	17.1	--
Amount of demineralized water / ml	100	100	100	100
Stirring time at 30 °C / h	24	48	72	72
pH at 24 °C	6.3	6.3	6.3	6.0
Dilution factor	1	1	1	1
Measured concentration / mg/L	< 1.014 / < 1.014	< 1.014 / < 1.014	< 1.014 / < 1.014	- / -
Mean concentration / mg/L	< 1.014	< 1.014	< 1.014	-
Water solubility / mg/L	< 1.0	< 1.0	< 1.0	-

The water solubility of the test item at 20 °C was determined to be below 1.0 mg/L.

Conclusions:

Water solubility (EU method A.6; flask method): <1.0 mg/L at 20 °C

Executive summary:

The water solubility of the test item WARADUR LG at a temperature of 20 °C was determined in a reliable study (RL 1) performed according to EU method A.6 compliant with GLP (flask method). Independently of the duration of the experiment (24 h, 48 h and 72 h, respectively at 30°C, followed by a 24 h equilibration at 20°C before measurement) it was found to be below the limit of quantification of the analytical method (TOC analysis).

Water solubility < 1.0 mg/L at 20°C

External to the study report, attached original measurement data for Waradur LG were evaluated. The lowest standard analysed for calculation of the calibration curve was 1.515 mg/L. The calibration curve was highly linear and of excellent fit (see plot attached). Therefore, extrapolation to lower calibration range seems to be acceptable. The reasoning to do so is because a "lower than value" is of no worth for assessment of environmental fate.

Attached to the report is the chromatogram including data evaluation of one measurement ("sample A") for Waradur LG. If samples were assigned numbering according to increasing incubation time, 24 h incubation at 30°C followed by 24 hours equilibration at 20°C would characterize this sample. Three replicate measurements were performed, distinct peaks were obtained, and SD (concentration) was low (0.00914 mg/L). The determined mean concentration is given as 0.5465 mg/L (single rounded values: 0.5530 mg/L; 0.5237 mg/L; 0.5401 mg/L).

As such, the approximate water solubility for Waradur LG relevant for environmental fate assessment is 0.55 mg/L.

Because this concentration refers to TOC analysis, due to oxygen being part of the substance the value based on MW will be somewhat higher.

Description of key information

Water solubility (EU method A.6; flask method): <1.0 mg/L at 20 °C

Key value for chemical safety assessment

Water solubility:

0.55 mg/L

at the temperature of:

20 °C

Additional information

The water solubility of the test item WARADUR LG at a temperature of 20 °C was determined in a reliable study (RL 1) performed according to EU method A.6 compliant with GLP (flask method). Independently of the duration of the experiment (24 h, 48 h and 72 h, respectively at 30°C, followed by a 24 h equilibration at 20°C before measurement) it was found to be below the limit of quantification of the analytical method (TOC analysis).

Water solubility < 1.0 mg/L at 20°C

External to the study report, original measurement data for Waradur LG included within the study report were evaluated. The lowest standard analysed for calculation of the calibration curve was 1.515 mg/L. The calibration curve was highly linear and of excellent fit. Therefore, extrapolation to lower calibration range seems to be acceptable. The reasoning to do so is because a "lower than value" is of no worth for assessment of environmental fate.

As an annex to the report is the chromatogram including data evaluation of one measurement ("sample A") for Waradur LG. If samples were assigned numbering according to increasing incubation time, 24 h incubation at 30°C followed by 24 hours equilibration at 20°C would characterize this sample. Three replicate measurements were performed, distinct peaks were obtained, and SD (concentration) was low (0.00914 mg/L). The determined mean concentration is given as 0.5465 mg/L (single rounded values: 0.5530 mg/L; 0.5237 mg/L; 0.5401 mg/L).

As such, the approximate water solubility for Waradur LG relevant for environmental fate assessment is 0.55 mg/L.

Because this concentration refers to TOC analysis, due to oxygen being part of the substance the value based on MW will be somewhat higher.