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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994-05-10 to 1994-05-12
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
(1984)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
signed 1994-03-16
Analytical monitoring:
yes
Details on sampling:
- Sampling method: Samples were taken of fresh and the 48-h old media at test end
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dispersion in water
- Controls: yes, one control
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus
- Source: laboratory culture
- Age of parental stock: gravid adults
- Feeding during test: none

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Adults were isolated 24 hours prior to test initiation and the overnight produced young daphnids were used for testing.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
270 mg/L as CaCO3
Test temperature:
21.0 °C
pH:
7.6 - 7.7
Dissolved oxygen:
at test start 8.4 mg/L
at test end 7.9 - 8.0 mg/L
Nominal and measured concentrations:
Nominal concentrations: 0 (control), 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56, and 100 mg test item/L
For measured concentrations please refer to "any other information on results incl. tables", since not all nominal concentrations were analytically verified at 0 hours.
Details on test conditions:
TEST SYSTEM
- Test vessel:glass jars containing 250 mL test solution
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 25 mL per organism

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (ISO) according to OECD 202
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: only if necessary to 7.8 ± 0.2
- Photoperiod: 16 h light and 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation


RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
42 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 %-CI: 36 - 51 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Analysis of the immobility data by the moving average method of Thompson (1947).

Cumulative Immobilisation

Nominal Concentration

(mg/L)

Cumulative immobilised Daphnia

(initial population: 10 per replicate)

24 hours

48 hours

R1

R2

Total

%

R1

R2

Total

%

Control

0

0

0

0

0

0

0

0

1.0

0

0

0

0

0

0

0

0

1.8

0

0

0

0

0

0

0

0

3.2

0

0

0

0

0

0

0

0

5.6

0

0

0

0

0

0

0

0

10

0

0

0

0

0

0

0

0

18

0

0

0

0

0

0

0

0

32

0

0

0

0

4

3

7

35

56

4

3

7

35

7

6

13

65

100

10

10

20

100

10

10

20

100

R1and R2= Replicates 1 and 2

Analytical results for the test item

Samples

Nominal concentration

(mg/L)

Concentration found

(mg/L)

Concentration

expressed as % of nominal

0 hours

Control

1.0

3.2

10

32

100

<L.O.D.

0.938

2.88

8.94

28.3

87.5

-

93.8

90.0

89.4

88.6

87.5

48 hours

Control

1.0

1.8

3.2

5.6

10

18

32

56

100

<L.O.D.

1.01

1.73

3.19

4.72

9.83

16.5

25.3

46.1

84.9

-

100.6

95.6

99.6

84.3

98.3

91.6

79.0

82.3

84.9

L.O.D. = Limit of detection

Validity criteria fulfilled:
yes
Conclusions:
The test item was found to immobilise Daphnia magna at concentrations higher than 18 mg/L after 48 hours. Based on the nominal test item concentrations, the 48-hour EC50 for Daphnia magna amounts to 42 mg/L (95 %-CI: 36 - 51 mg/L).
Executive summary:

The toxic effect of the test item to Daphnia magna was examined according to OECD 202 (1984) under static conditions over a period of 48 hours. For the determination of the test concentrations, a range-finding test was performed. In the definitive test, each test concentration and the control was tested in duplicate with ten daphnids each. Effect concentrations were calculated based on nominal test concentration since analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The corresponding 48-hour EC50 value was estimated with 42 mg/L (95 %-CI: 36 - 51 mg/L).

Description of key information

The test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The 48-hour EC50 value was estimated with 42 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
42 mg/L

Additional information

The toxic effect of the test item to Daphnia magna was examined according to OECD 202 (1984) under static conditions over a period of 48 hours. For the determination of the test concentrations, a range-finding test was performed. In the definitive test, each test concentration and the control was tested in duplicate with ten daphnids each. Effect concentrations were calculated based on nominal test concentration since analytical recoveries were in the range of 80 to 120 % throughout the test. After 48 hours of exposure the test item was found to immobilise Daphnia magna at concentrations above 18 mg/L (NOEC). The corresponding 48-hour EC50 value was estimated with 42 mg/L (95 %-CI: 36 - 51 mg/L).