EPOFLOC L-1R

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28th - 30th January 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Astra Zeneca, Brixham
- Age at study initiation (mean and range, SD): Less than 24 hours
- Feeding during test: The daphnia were not fed during the test
- Food type: a suspension of Chlorella vulgaris
- Amount: rate of 1 mg organic carbon/litre of culture water
- Frequency: Each working day until 24 hours before test initiation

ACCLIMATION
- No details provided

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

No data

Test temperature:

19.5 - 20.2°C

pH:

7.36 - 8.46

Dissolved oxygen:

6.96 - 9.18 mg/l

Nominal and measured concentrations:

0 (Control), 6.3, 12.5, 25, 50 and 100 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: 60 ml disposable plastic vessel
- Type (delete if not applicable): closed - to limit aerial contamination and replace evaporative losses, a transparent Perspex sheet was placed over the tops of the dishes
- Material, size, headspace, fill volume: Test volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Test preformed in Elendt M4 medium.
- Intervals of water quality measurement: pH, dissolved oxygen and temperature measured at start of test and end of 48 hour test period. Temperature was recorded at 0, 24 and 48 hours.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): 24 and 48 hour EC50.

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study:
Preliminary study undertaken at concentrations of 0, 0.1, 1, 10 and 100 mg/l. Data from the preliminary study identified the 48 hour EC50 as being approximately between 10 and 100 mg/l.

Exposure period (hours) Cumulative percentage immobilisation
Concentration mg/l
0 0.1 1 10 100
24 0 0 0 0 0
48 0 0 0 0 5

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

64.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits: 54.8 to 77.0 mg/l

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Dunnet's test

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Dunnet's test.

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

70.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits: 67.6 to 75.5 mg/l

Details on results:

- Mortality of control: None

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/LC50:
- Results of inter laboratory tests and a Technical Corrigendum to ISO 6341: 24h EC50 0.6 mg/l to 2.1 mg/l.
- A reference study was run outside of this study: 24h EC50 1.067 mg/l.

Reported statistics and error estimates:

Where possible the EC50 values are shown graphically and calculated with 95% confidence limits according to the methods of ToxCalcTM Version 5.0 ‘’Comprehensive Toxicity Data Analysis and Database Software’’, copyright 1994-1996.

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 value was determined to be 64.3 mg/l.

Description of key information

The 48h EC50 value was determined to be 64.3 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

64.3 mg/L