Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28th - 30th January 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: Astra Zeneca, Brixham
- Age at study initiation (mean and range, SD): Less than 24 hours
- Feeding during test: The daphnia were not fed during the test
- Food type: a suspension of Chlorella vulgaris
- Amount: rate of 1 mg organic carbon/litre of culture water
- Frequency: Each working day until 24 hours before test initiation

ACCLIMATION
- No details provided
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
No data
Test temperature:
19.5 - 20.2°C
pH:
7.36 - 8.46
Dissolved oxygen:
6.96 - 9.18 mg/l
Nominal and measured concentrations:
0 (Control), 6.3, 12.5, 25, 50 and 100 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel: 60 ml disposable plastic vessel
- Type (delete if not applicable): closed - to limit aerial contamination and replace evaporative losses, a transparent Perspex sheet was placed over the tops of the dishes
- Material, size, headspace, fill volume: Test volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Test preformed in Elendt M4 medium.
- Intervals of water quality measurement: pH, dissolved oxygen and temperature measured at start of test and end of 48 hour test period. Temperature was recorded at 0, 24 and 48 hours.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): 24 and 48 hour EC50.

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study:
Preliminary study undertaken at concentrations of 0, 0.1, 1, 10 and 100 mg/l. Data from the preliminary study identified the 48 hour EC50 as being approximately between 10 and 100 mg/l.

Exposure period (hours) Cumulative percentage immobilisation
Concentration mg/l
0 0.1 1 10 100
24 0 0 0 0 0
48 0 0 0 0 5
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
64.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limits: 54.8 to 77.0 mg/l
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Dunnet's test
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Dunnet's test.
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
70.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limits: 67.6 to 75.5 mg/l
Details on results:
- Mortality of control: None
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- EC50/LC50:
- Results of inter laboratory tests and a Technical Corrigendum to ISO 6341: 24h EC50 0.6 mg/l to 2.1 mg/l.
- A reference study was run outside of this study: 24h EC50 1.067 mg/l.
Reported statistics and error estimates:
Where possible the EC50 values are shown graphically and calculated with 95% confidence limits according to the methods of ToxCalcTM Version 5.0 ‘’Comprehensive Toxicity Data Analysis and Database Software’’, copyright 1994-1996.
Validity criteria fulfilled:
yes
Conclusions:
The 48h EC50 value was determined to be 64.3 mg/l.

Description of key information

The 48h EC50 value was determined to be 64.3 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
64.3 mg/L

Additional information

Categories Display