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EC number: - | CAS number: -
- Life Cycle description
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- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Biotransformation and kinetics
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
- Carcinogenicity
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- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20th - 24th January 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Study Plan specified the study will be performed in a temperature controlled lab at 22 ± 2°C. The test temperature was recorded at 19.0 - 20.5°C. In the opinion of the Study Director this deviation had no effect on the outcome of the study.
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF 2-7-1, Acute Fish Toxicity
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Source: Hampshire Carp Hatcheries, Bowlake Fish Farm, 5 Segars Lane, Twyford, Winchester, Hampshire, SO21 1QJ, UK.
ACCLIMATION
- Acclimation period: 11 October 2013 - 20 January 2014
- Acclimation conditions (same as test or not): Temperature: 20.0 - 21.0°C; pH: 7.89 - 8.18; Dissolved oxygen: 85 - 98% ASV; Photoperiod: 16 hours light and 8 hours dark
- Type and amount of food: Nutrafin Max Goldfish Flakes
- Feeding frequency: daily at a rate of approximately 1-2% body weight per day until 24 hours before test initiation. Not fed for the duration of study.
- Health during acclimation (any mortality observed): 0% - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- N/A
- Hardness:
- Sample from control vessel: 228 mg CaCO3/l
- Test temperature:
- Temperature of stock tank water at test initiation: 20.0°C
Temperature values recorded during test (°C): 19.0 - 20.5 - pH:
- pH of stock tank water at test initiation: 7.99
pH values recorded during test: 7.57 - 8.74 - Dissolved oxygen:
- Dissolved oxygen of stock tank water at test initiation: 96% ASV
Dissolved oxygen values recorded during test: 86 - 100% - Salinity:
- N/A
- Nominal and measured concentrations:
- 0 (control), 10, 26, 64, 160, 400 and 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All-glass aquaria of length 45.5cm x height 26cm x width 25.5cm
- Material, size, headspace, fill volume: Test volume: 20 litres
- Renewal rate of test solution (frequency/flow rate): test solutions replaced every day, the remaining live fish being carefully transferred to freshly prepared test solutions by net.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: The stocks of animals were maintained, and the tests performed, in a mixture of dechlorinated mains water and Reverse Osmosis (R.O.) treated mains water at a ratio of 80%:20%.
- Intervals of water quality measurement: pH, dissolved oxygen and temperature were measured immediately prior to initiating the test, before and after each daily change of test solutions and at the end of the 96 hour exposure period.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): 24h, 48, 72h and 96h LC50; 24h, 48, 72h and 96h NOEC
TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: Data supplied from the Sponsor on the toxicity of the test substance to fish indicated the NOEC was 10 mg/l and the 96h LC50 was >1000 mg/l. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Sub-lethal effects
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Sub-lethal effects
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Sub-lethal effects
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Sub-lethal effects
- Reported statistics and error estimates:
- Statistical analysis of the mortality data using Toxcalc was not carried out.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study Epofloc L-1R was not found to be toxic.
Reference
Description of key information
The LC50 was determined to be > 1000 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
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