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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22nd January 2014 to 20th February 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Cambridge STW, Cowley Road, Cambridge
- Pretreatment: Filtered (Whatman No. 54)
- Concentration of sludge: 1 ml/l
- Date of collection: 23 January 2014
- Temperature at start of test: 19.7°C - Duration of test (contact time):
- 28 d
- Initial conc.:
- 11.48 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The test material was insufficiently defined to allow the use of ThOD as a comparator for ready biodegradability. An experimental COD measure was used instead.
TEST CONDITIONS
- Composition of medium: Mineral nutrient stock solutions added to deionised water and aerated for 20 minutes. Left to stand for 23 hours and 40 minutes. Inoculum then added.
- Test temperature: 19.5 - 21.2°C
- Continuous darkness: Yes
TEST SYSTEM
- Number of culture flasks/concentration: 1 replicate for day 0, 2 replicates for days 7 and 14 and 3 replicates for days 21 and 28.
- Measuring equipment: Dissolved oxygen meter
- Test performed in open system: No
SAMPLING
- Sampling frequency: 0, 7, 14, 21 and 28 days
- Sampling method: Dissolved oxygen meter
- Sample storage before analysis: Room temperature
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes - Reference substance:
- other: sodium acetate
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 18
- Sampling time:
- 28 d
- Remarks on result:
- other: Average degradation (calculated from replicates 29%, 12% and 11% degradation)
- Parameter:
- % degradation (O2 consumption)
- Value:
- 18
- Sampling time:
- 21 d
- Remarks on result:
- other: Average degradation (calculated from replicates 29%, 12% and 11% degradation)
- Parameter:
- % degradation (O2 consumption)
- Value:
- 20
- Sampling time:
- 14 d
- Remarks on result:
- other: Average degradation (calculated from replicates 29% and 11% degradation)
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7
- Sampling time:
- 7 d
- Remarks on result:
- other: Average degradation (calculated from replicates 5% and 10% degradation)
- Key result
- Parameter:
- COD
- Value:
- 566 mg O2/g test mat.
- Results with reference substance:
- Average degradation
7 days: 66%
14 days: 74%
21 days: 71%
28 days: 80% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Epofloc L-1R failed to meet the criteria for classification as a readily biodegradable substance
- Executive summary:
- Test commissioned by: Miyoshi Oil and Fat Co., Ltd
- Test type: Ready Biodegradability (Closed Bottle test)
- Test period: 22 to 23 January 2004 (COD analysis); 23 January to 20 February 2014 (OECD 301D)
- Test carried out at: Chemex Environmental International Limited, Unit J, Broad Lane Industrial Estate, Cottenham, Cambridge, CB23 8SW, UK.
- Results:
Time (days)
% Biodegradation
Reference material
Test substance*
7
66
7
14
74
20
21
71
18
28
80
18
*Values are for a test concentration of 11.48 mg/l and a Chemical Oxygen Demand (COD) of 566 mgO2/g.
Reference
ThOD of sodium acetate 780 mg O2/g
Description of key information
This substance was found to be not biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
There was limited biodegradation with 18% recorded after 28 days.
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