EPOFLOC L-1R

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22nd January 2014 to 20th February 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Cambridge STW, Cowley Road, Cambridge
- Pretreatment: Filtered (Whatman No. 54)
- Concentration of sludge: 1 ml/l
- Date of collection: 23 January 2014
- Temperature at start of test: 19.7°C

Duration of test (contact time):

28 d

Initial conc.:

11.48 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The test material was insufficiently defined to allow the use of ThOD as a comparator for ready biodegradability. An experimental COD measure was used instead.

TEST CONDITIONS
- Composition of medium: Mineral nutrient stock solutions added to deionised water and aerated for 20 minutes. Left to stand for 23 hours and 40 minutes. Inoculum then added.
- Test temperature: 19.5 - 21.2°C
- Continuous darkness: Yes

TEST SYSTEM
- Number of culture flasks/concentration: 1 replicate for day 0, 2 replicates for days 7 and 14 and 3 replicates for days 21 and 28.
- Measuring equipment: Dissolved oxygen meter
- Test performed in open system: No

SAMPLING
- Sampling frequency: 0, 7, 14, 21 and 28 days
- Sampling method: Dissolved oxygen meter
- Sample storage before analysis: Room temperature

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes

Reference substance:

other: sodium acetate

Key result

Parameter:

% degradation (O2 consumption)

Value:

18

Sampling time:

28 d

Remarks on result:

other: Average degradation (calculated from replicates 29%, 12% and 11% degradation)

Parameter:

% degradation (O2 consumption)

Value:

18

Sampling time:

21 d

Remarks on result:

other: Average degradation (calculated from replicates 29%, 12% and 11% degradation)

Parameter:

% degradation (O2 consumption)

Value:

20

Sampling time:

14 d

Remarks on result:

other: Average degradation (calculated from replicates 29% and 11% degradation)

Parameter:

% degradation (O2 consumption)

Value:

7

Sampling time:

7 d

Remarks on result:

other: Average degradation (calculated from replicates 5% and 10% degradation)

Key result

Parameter:

COD

Value:

566 mg O2/g test mat.

Results with reference substance:

Average degradation
7 days: 66%
14 days: 74%
21 days: 71%
28 days: 80%

ThOD of sodium acetate 780 mg O₂/g

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Epofloc L-1R failed to meet the criteria for classification as a readily biodegradable substance

Executive summary:

- Test commissioned by: Miyoshi Oil and Fat Co., Ltd

- Test type: Ready Biodegradability (Closed Bottle test)

- Test period: 22 to 23 January 2004 (COD analysis); 23 January to 20 February 2014 (OECD 301D)

- Test carried out at: Chemex Environmental International Limited, Unit J, Broad Lane Industrial Estate, Cottenham, Cambridge, CB23 8SW, UK.

- Results:

 

Time (days)	% Biodegradation
	Reference material	Test substance*
7	66	7
14	74	20
21	71	18
28	80	18

 

*Values are for a test concentration of 11.48 mg/l and a Chemical Oxygen Demand (COD) of 566 mgO₂/g.

Description of key information

This substance was found to be not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater

Additional information

There was limited biodegradation with 18% recorded after 28 days.