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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22nd January 2014 to 20th February 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Cambridge STW, Cowley Road, Cambridge
- Pretreatment: Filtered (Whatman No. 54)
- Concentration of sludge: 1 ml/l
- Date of collection: 23 January 2014
- Temperature at start of test: 19.7°C
Duration of test (contact time):
28 d
Initial conc.:
11.48 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The test material was insufficiently defined to allow the use of ThOD as a comparator for ready biodegradability. An experimental COD measure was used instead.

TEST CONDITIONS
- Composition of medium: Mineral nutrient stock solutions added to deionised water and aerated for 20 minutes. Left to stand for 23 hours and 40 minutes. Inoculum then added.
- Test temperature: 19.5 - 21.2°C
- Continuous darkness: Yes

TEST SYSTEM
- Number of culture flasks/concentration: 1 replicate for day 0, 2 replicates for days 7 and 14 and 3 replicates for days 21 and 28.
- Measuring equipment: Dissolved oxygen meter
- Test performed in open system: No

SAMPLING
- Sampling frequency: 0, 7, 14, 21 and 28 days
- Sampling method: Dissolved oxygen meter
- Sample storage before analysis: Room temperature

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
Reference substance:
other: sodium acetate
Key result
Parameter:
% degradation (O2 consumption)
Value:
18
Sampling time:
28 d
Remarks on result:
other: Average degradation (calculated from replicates 29%, 12% and 11% degradation)
Parameter:
% degradation (O2 consumption)
Value:
7
Sampling time:
7 d
Remarks on result:
other: Average degradation (calculated from replicates 5% and 10% degradation)
Parameter:
% degradation (O2 consumption)
Value:
20
Sampling time:
14 d
Remarks on result:
other: Average degradation (calculated from replicates 29% and 11% degradation)
Parameter:
% degradation (O2 consumption)
Value:
18
Sampling time:
21 d
Remarks on result:
other: Average degradation (calculated from replicates 29%, 12% and 11% degradation)
Key result
Parameter:
COD
Value:
566 mg O2/g test mat.
Results with reference substance:
Average degradation
7 days: 66%
14 days: 74%
21 days: 71%
28 days: 80%

ThOD of sodium acetate 780 mg O2/g

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Epofloc L-1R failed to meet the criteria for classification as a readily biodegradable substance
Executive summary:

- Test commissioned by: Miyoshi Oil and Fat Co., Ltd

- Test type: Ready Biodegradability (Closed Bottle test)

- Test period: 22 to 23 January 2004 (COD analysis); 23 January to 20 February 2014 (OECD 301D)

- Test carried out at: Chemex Environmental International Limited, Unit J, Broad Lane Industrial Estate, Cottenham, Cambridge, CB23 8SW, UK.

- Results:

 

Time (days)

% Biodegradation

Reference material

Test substance*

7

66

7

14

74

20

21

71

18

28

80

18

 

*Values are for a test concentration of 11.48 mg/l and a Chemical Oxygen Demand (COD) of 566 mgO2/g.

Description of key information

This substance was found to be not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

There was limited biodegradation with 18% recorded after 28 days.

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