Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 232-601-0 | CAS number: 9001-37-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 February 1992 to 3 December 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guideline No. 202, Part 1 and EEC Directive 67/548 Annex 5, C2 as published in 84/449/EEC
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 92.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: enyzme concentrate dry matter
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: enzyme concentrate dry matter
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- Analysis of the immobility of Daphnia magna exposed to peroxidase to concentrations up to 546.6 mg enzyme concentrate dry matter/L for 48 hours gave an EC50 value of 92.7 mg enzyme concentrate dry matter/L, equivalent to 26.2 mg/L active enzyme protein.
- Executive summary:
The acute toxicity of Peroxidase to Daphnia magna was assessed under static exposure conditions.
The study was conducted in accordance with OECD Guideline No. 202, Part 1 and EEC Directive 67/548 Annex 5, C2 as published in 84/449/EEC. A group of twenty Daphnia ≤24 hours old was exposed for 48 hours to Peroxidase in concentrations up to 546.6 mg enzyme concentrate dry matter/L. Dechlorinated tap water was used as test water, having a total hardness of 150 -200 mg/L.
Observations of the Daphnia in each control and test vessel were made after 24 and 48 hours, which produced these findings:
48-hour EC50 value: 92.7 mg/L (26.2 mg/L active enzyme protein)
48-hour NOEC value: 31.2 mg/L (8.8 mg/L active enzyme protein)
Reference
Description of key information
The short-term toxicity to aquatic invertebrates of the present target substance, Glucose oxidase IUBMB 1.1.3.4, has not been determined but another enzyme (Peroxidase, IUBMB 1.11.1.7) has been analyzed and used as a source substance for read-across. Analysis of the immobility of Daphnia magna exposed to peroxidase to concentrations up to 546.6 mg enzyme concentrate dry matter/L for 48 hours gave an EC50 value of 92.7 mg enzyme concentrate dry matter/L, equivalent to 26.2 mg/L active enzyme protein.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 92.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
