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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
no
Remarks:
not GLP study

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
EC Number:
304-762-8
EC Name:
Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
Cas Number:
94279-16-0
Molecular formula:
C40H24N10Na4O19S4
IUPAC Name:
tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): Cutlure Collection Algae Protozoo
- Method of cultivation:
temperature: 22+/-2°C,
Light intesity2500Lux
agitation: continuos

Study design

Test type:
static
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22+/-2°C
Nominal and measured concentrations:
1, 2.2, 4.8, 10, 22, 48 and 100 mg/l active material
Details on test conditions:
TEST SYSTEM
- Test vessel:glass tubes-40ml
- Type (delete if not applicable): closed
- Renewal rate of test solution (frequency/flow rate): every 48h
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2


OTHER TEST CONDITIONS
- Light intensity and quality: 2500 Lux

Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
IC50
Effect conc.:
ca. 12.8 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Results with reference substance (positive control):
IC50 72h = 1.1 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The substance was tested for acute toxicity to algae following OECD 201. Under the experimental conditions the IC50,72h is equal to 12.8 mg/l.
Executive summary:

The substance was tested for acute toxicity to algae following OECD 201. Under the experimental conditions the IC50,72h is equal to 12.8 mg/l.

The substance showed toxic effects to daphnia and needs to be classified under Regulation 1272/2008 (CLP) as Aq. Chronic 3, H412.

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