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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
EC Number:
304-762-8
EC Name:
Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
Cas Number:
94279-16-0
Molecular formula:
C40H24N10Na4O19S4
IUPAC Name:
tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Remarks:
water
Details on test solutions:
The mother solution was prepared dissolving 1,5385 g of active substance in 1000 ml of water for a final concentration of 1008,2 mg/l
The stock solutions were prepared from the mother solution.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Supplier: P & S, S.L
Lenght (cm): 2.86 ± 0,18)
Weight (g): 0.29 ± 0,12

Temperature: 22 °C ± 2
Photoperiod: 12 h light/ 12 h dark
Food: commercial feed fpr fish
Frequency: 3 times/week
Acclimatation period: minim 12 days
Mortality in acclimation period: 0%


Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
120 mg CaCO3/L
Test temperature:
22 °C ± 2
pH:
8,11
Dissolved oxygen:
> 70%
Nominal and measured concentrations:
0,10 mg/L, 1,01 mg/L, 10,01 mg/L and 100,8 mg/L of active indredient
Details on test conditions:
Fish tank volume 3L
1 tank for concentration
7 fish for tank
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The results show no toxicity on fish.
Executive summary:

The test performed on the tested item shows no toxicity on the test organisms with a LC50 > 100 mg/l.

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