α-d-Glucopyranoside, β-d-fructofuranosyl, mono, di, and tridodecanoate esters

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 8 - December 13, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Test material form:

solid: flakes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsubishi-Kagaku Foods Corporation
- Expiration date of the lot/batch: March 27, 2018
- Purity test date: October 14, 2016


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: < 15 degrees C, protected from light

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Remarks:

66% urban, 34% industrial

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): San Rocco - Monza
- Storage conditions: aerobic
- Preparation of inoculum for exposure: The muds were allowed to mix and settle. They were then centrifuged, washed, and analyzed.
- Initial cell/biomass concentration: 4 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

20.38 g/L

Based on:

TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

inorg. C analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: 10 mL of a solution containing potassium dihydrogenphosphate (8.50 g/L), dipotassium hydrogenphosphate (21.75 g/L), disodium hydrogenphosphate dihydrate (33.40 g/L), ammonium chloride (0.50 g/L); 1 mL of a solution containing calcium chloride dihydrate (36.40 g/L); 1 mL of solution containing magnesium sulfate heptahydrate (22.50 g/L); and 1 mL of solution containing iron chloride (III) hexahydrate (0.25 g/L)
- Test temperature: 20 +/- 2 degrees C
- Suspended solids concentration: 4 mg/L
- Continuous darkness: yes
- Other: stirring

TEST SYSTEM
- Culturing apparatus: bottles
- Number of culture flasks/concentration: 17 (11 with test substance and reference substance)
- Measuring equipment: TOC analyzer with an infrared gas analyzer
- Test performed in closed vessels: yes

SAMPLING
- Sampling frequency: Days 0, 1, 4, 14, 21, and 28
- Sampling method: 12 hrs prior, the reaction in one bottle would be stopped with addition of 6 mL 1 M sodium hydroxide.
- Sample storage before analysis: samples were diluted with deionized water


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (inorg. C analysis)

Value:

6

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (inorg. C analysis)

Value:

54

Sampling time:

4 d

% Degradation 3

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

78

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (inorg. C analysis)

Value:

80

Sampling time:

21 d

% Degradation 5

Parameter:

% degradation (inorg. C analysis)

Value:

102

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance biodegraded 99% in 14 days. The test was therefore considered valid.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The substance LMDT is readily biodegradable.

Executive summary:

LMDT was tested for biodegradability in an OECD guideline 301 test. LMDT biodegraded 78% in 14 days, and 102% degraded in 28 days. LMDT is readily biodegradable.