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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06 February 2017 to 08 March 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: in the main test controls and at 100 mg/L at 0 hour (bulk) and 48 hour (pooled from replicates) in duplicate
- Sample storage conditions before analysis:frozen
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION : A nominal amount of test item (100 mg) was dissolved in a 1000 mL volume of test water with the aid of sonication for approximately 5 minutes to give the 100 mg/L test concentration.
The prepared concentration was inverted several times to ensure adequate mixing and homogeneity.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house laboratory culture
- Age : < 24 hours
- Feeding during test: none

ACCLIMATION
- Acclimation period: NA
- Culture conditions: temperature 18 to 22 °C; 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.:
- Type and amount of food: algal suspension (Desmodesmus subspicatus) and Tetramin flake food suspensio
- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
NA
Test temperature:
19-22 ºC
pH:
7.7-7.8
Dissolved oxygen:
8.5-9.2 mg/L (102-108% of saturation)
Salinity:
NA
Nominal and measured concentrations:
nominal: 0 and 100 mg/L
measured (at 0 and 48 h):
Details on test conditions:
TEST SYSTEM
- Test vessel: 150 mL glass beakers with 100 mL test preparation
- Type: closed
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: fresh water (Elendt M7 medium according to the guideline pH 7.9, kept at 21 ºC)
- Adjustment of pH: no
- Photoperiod: 16 h light
- Light intensity: 386 to 568 Lux

EFFECT PARAMETERS MEASURED: immobility:

RANGE-FINDING STUDY
- Test concentrations: 0, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study:no immobilisation at any of the concentrations tested
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: measured concentrations >80% of nominal
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: measured concentrations >80% of nominal
Results with reference substance (positive control):
potassium dichromate EC50 (48 h) 1.2 mg/L
Reported statistics and error estimates:
NA
Validity criteria fulfilled:
yes
Conclusions:
The 48 h EC50 for daphnia magna is >100 mg/L
Executive summary:

In a static test according to OECD 202 20 daphnids/concentration were exposed to 0 and 100 mg/L in Elendt M7 medium. Measured concentrations at 0 and 100 mg/L were at 0 and 48 h: <LOQ and < LOQ, and 98.0 and 96.3 mg/L. No immobility was observed. The 48 h EC50 for daphnia magna is > 100 mg/L.

Description of key information

In a static test according to OECD 202 20 daphnids/concentration were exposed to 0 and 100 mg/L in Elendt M7 medium. Measured concentrations at 0 and 100 mg/L were at 0 and 48 h: <LOQ and < LOQ, and 98.0 and 96.3 mg/L. No immobility was observed. The 48 h EC50 for daphnia magna is > 100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information