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EC number: 249-648-8 | CAS number: 29461-13-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- WoE report is prepared based on short term toxicity to aquatic invertebrates studies
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Remarks:
- In first study analytical monitoring was done
- Vehicle:
- not specified
- Details on test solutions:
- WoE-2 Method: Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium.
WoE-3: The stock solution (150 mg/L) was prepared by dissolving colourless liquid in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. It was not possible to prepare homogenous solution of higher concentration. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. - Test organisms (species):
- other: 1. Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum), 2. Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- WoE-2 :Details on test organisms
TEST ORGANISM
- Common name: Green algae
WoE-3:
TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- WoE-2: 22.3 - 22.6 ° C
WoE-3: 23 ± 2°C - pH:
- WoE-3: Without adjustment,
The sample at concentration 150 mg/l: pH= 7.7 did not changed during the test,
Control: pH= 8.1 changed to 7.9 during the test - Nominal and measured concentrations:
- WoE-2: 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control
WoE-3 : 0, 30, 45, 67, 100 and 150 mg/L - Details on test conditions:
- WoE-2: TEST SYSTEM:
- Test vessel: Erlenmeyer flask
- Exposure system: rotary shaking culture (ca. 100 rpm),
- Material, size, headspace, fill volume: test solution volume; 600 mL/control (100 mL/vessel), 300 mL/ concentration (100 mL/vessel), (Separately, a vessel was reserved for analysis at 48 hours.)
- No. of vessels per concentration (replicates): 3 (Separately from that, another vessel for analysis at 48 hours was prepared.)
- No. of vessels per control (replicates):6
EFFECT PARAMETERS MEASURED:
- Measurement of biomass: Cell concentration
OTHER TEST CONDITIONS:
- Light intensity and quality: 98 - 99 microE/m2/s
WoE-3:
TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- for WoE-3: Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % CL: 220 - 270 mg/L
- Remarks:
- 1st study
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 172.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 170.7 - 175.1 mg/l
- Validity criteria fulfilled:
- not specified
- Conclusions:
- After exposure of test chemical to aquatic algaefor 72 hrs the EC 50 value of test chemical is expected to be in range from 172.9 to 250 mg/l. By considering this range EC 50 value test chemical is considered to be non toxic and not classified as per the CLP classification criteria.
- Executive summary:
Short term toxicity of test chemical on the growth of aquatic algae was carried out on the basis of it structurally and functionally similar read across chemicals. The results of both the studies are as mentioned below:
Freshwater algal growth inhibition test was carried out according to OECD Guideline 201. The test organism used in this study was on Desmodesmus subspicatus .The stock solution (150 mg/L) of test chemical was prepared by dissolving test chemical in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 30, 45, 67, 100 and 150 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test chemical, in Desmodesmus subspicatus was determined to be 172.9 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and can be considered as not classified as per the CLP criteria.
In another study the Growth Inhibition of Alga (Pseudokirchneriella subcapitata) was determined by the exposing the test chemical for 72 hrs. Test conducted according to OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test). Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium. Test was conducted at the different concentrations 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control. Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure, after 4 hours from start of exposure, and at the end of exposure. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to exposure of test chemical for 72 hrs, the effect concentration EC50 and NOEC value was determined to be 250 mg/l and 16 mg/l respectively. Thus on the basis of EC50, test chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.
Thus based on the results of both the studies for test chemical, it can be concluded that EC 50 value of test chemical is expected to be range from 172.9 to 250 mg/l. By considering this range EC 50 value test chemical is considered to be non toxic and not classified as per the CLP classification criteria.
Reference
Description of key information
After exposure of test chemical to aquatic algaefor 72 hrs the EC 50 value of test chemical is expected to be in range from 172.9 to 250 mg/l. By considering this range EC 50 value test chemical is considered to be non toxic and not classified as per the CLP classification criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 250 mg/L
Additional information
Short term toxicity of test chemical on the growth of aquatic algae was carried out on the basis of it structurally and functionally similar read across chemicals. The results of both the studies are as mentioned below:
Freshwater algal growth inhibition test was carried out according to OECD Guideline 201. The test organism used in this study was on Desmodesmus subspicatus .The stock solution (150 mg/L) of test chemical was prepared by dissolving test chemical in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 30, 45, 67, 100 and 150 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test chemical, in Desmodesmus subspicatus was determined to be 172.9 mg/L. This value indicates that the substance is likely to be non-hazardous to aquatic algae and can be considered as not classified as per the CLP criteria.
In another study the Growth Inhibition of Alga (Pseudokirchneriella subcapitata) was determined by the exposing the test chemical for 72 hrs. Test conducted according to OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test). Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by adding and stirring the substance under the water surface of the Erlenmeyer flask filled with medium. Test was conducted at the different concentrations 500, 160, 50, 16, 5.0 mg/L (geometric ratio; square root of 10), control. Analytical monitoring was also done using the GC were the Frequency of analysis was at the start of exposure, after 4 hours from start of exposure, and at the end of exposure. Based on the growth rate inhibition of test organism Pseudokirchneriella subcapitata due to exposure of test chemical for 72 hrs, the effect concentration EC50 and NOEC value was determined to be 250 mg/l and 16 mg/l respectively. Thus on the basis of EC50, test chemical was consider as nontoxic and not classified as toxic as per the CLP classification criteria.
Thus based on the results of both the studies for test chemical, it can be concluded that EC 50 value of test chemical is expected to be value range from 172.9 to 250 mg/l. By considering this range EC 50 value test chemical is considered to be nontoxic and not classified as per the CLP classification criteria.
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