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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
7 June - 26 July 2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Appearance: Clear, colourless liquid
Storage conditions: Room temperature in the dark
Batch number: 52834
Expiry date: December 2006
Purity: 99%
Analytical monitoring:
yes
Details on sampling:
On each occasion, the test substance was dissolved in a volumetric flask containing diluent water. The contents of the flask were shaken before being poured into a test vessel (glass aquarium) containing diluent water and the volume adjusted to 25 litres with water.
Four, mid-vessel samples (100 ml) of medium were taken from the control and test vessels at 0 and 72 hours (fresh medium) and at 24 and 96 hours (expired medium) for analysis. On each occasion, the samples were stored in a refrigerator for up to two days before they were analysed; the others were stored in a refrigerator in case further analysis was required.
Vehicle:
no
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Dechlorinated tap water
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Age at study initiation (mean and range, SD): eggs hatched in March 2005
- Length at study initiation (length definition, mean, range and SD): 5.43 cm
- Weight at study initiation (mean and range, SD): 2.38 g
- Maintenance of the brood fish: aerated supply of diluent water under flow-through conditions

ACCLIMATION
- Acclimation period: 14 days
- Type and amount of food during acclimation: commercial fish food, 2.5% of the total wet-weight of fish
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): <1%

No food was given during the 21-hour period immediately before exposure or during the exposure period itself. No medication was given during the holding period
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
CA 180 mg CaCO3/L
Test temperature:
15.5 ± 0.5°C
pH:
7.56 - 8.31
Dissolved oxygen:
81 - 99 % air saturation value
Conductivity:
350 µS/cm (20°C)
Nominal and measured concentrations:
nominal concentration: 100 mg/l
measured concentration: 103 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquarium
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 25 litres of medium to a depth of 17 cm
- Aeration: yes
- No. of organisms per vessel: 1
- Biomass loading rate: initial static loading of 0.95 g bodyweight/litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered dechlorinated tap water blended with tap water that had been softened

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light: 8 hours dark with periods of subdued lighting at the beginning and end of each light phase

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : death, absence of respiratory movement, absence of response to physical stimulation of the caudal peduncle

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 10 and 100 mg/l
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 103 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 103 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The measurements of water quality (temperature, pH, concentrations of dissolved oxygen and total hardness) remained within acceptable limits throughout the study.
Validity criteria fulfilled:
yes
Conclusions:
No mortality or treatment-related effects on the fish were exhibited during the study.
Based on these findings the following values have been estimated:
96-hour LC50 value : > 103 mg/l.
“No observed effect concentration” : > 103 mg/l.

Description of key information

96-hour LC50 value : > 103 mg/l.

“No observed effect concentration” : > 103 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
103 mg/L

Additional information